Zand Vahid, Binesh Fariba, Meybodian Mojtaba, Safi Dahaj Farzan, Alamdar Yazdi Arezoo
Dept. of Otolaryngology, Head and Neck Surgery, Otorhinolaryngology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Dept. of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Iran J Pathol. 2020 Summer;15(3):245-250. doi: 10.30699/ijp.2020.116579.2276. Epub 2020 May 1.
BACKGROUND & OBJECTIVE: Laryngeal squamous cell carcinoma (LSCC) is considered to be one of the most common cancers of the head and neck, accounting for roughly 90% of all malignant tumors of the larynx. To have a timely diagnosis for a better and practical therapy, molecular markers have to be investigated. The aim of this study was to determine the expression of Cyclin D1 (CD1) in patients with laryngeal squamous cell carcinoma.
In this study the demographic data of 82 patients with laryngeal squamous cell carcinoma, including age, gender and geographical region history of smoking and drug abuse, paraclinical findings, surgical description, and pathologic reports were extracted from their medical records. The stage and grade of the disease and tumor location were determined using their medical records. An appropriate tissue sample was selected. Then, the selected cancerous tissue samples stored as formalin-fixed paraffin-embedded tissue then were (Immunohistochemistry) IHC stained and analyzed in terms of the expression of CD1.
RESULTS & CONCLUSION: According to the results, 75 out of 82 (91.5%) investigated samples were positive for CD1 expression. There was a significant relationship between stage of the disease (=0.041) and CD1 expression in patients with laryngeal squamous cell carcinoma. There was no significant relationship between gender (=0.055), age (=0.256), history of smoking and drug abuse (=0.192), location of the tumor (=0.90), grade of the disease (=0.515) and geographical region (=0.466) and CD1 expression in patients with laryngeal squamous cell carcinoma. The results of the present study showed that CD1 expression was higher (91.5%) in patients with laryngeal squamous cell carcinoma in comparison to the other studies. According to the results we can conclude that stage of the disease can significantly affect CD1 expression in patients with squamous cell carcinoma.
喉鳞状细胞癌（LSCC）被认为是头颈部最常见的癌症之一，约占喉部所有恶性肿瘤的90%。为了能及时诊断以便进行更好且切实可行的治疗，必须对分子标志物进行研究。本研究的目的是确定细胞周期蛋白D1（CD1）在喉鳞状细胞癌患者中的表达情况。
在本研究中，从82例喉鳞状细胞癌患者的病历中提取了人口统计学数据，包括年龄、性别、地理区域、吸烟和药物滥用史、临床旁检查结果、手术描述和病理报告。根据病历确定疾病的分期、分级和肿瘤位置。选取合适的组织样本。然后，将选取的癌组织样本储存为福尔马林固定石蜡包埋组织，接着进行免疫组织化学（IHC）染色，并分析CD1的表达情况。
根据结果，82例被调查样本中有75例（91.5%）CD1表达呈阳性。喉鳞状细胞癌患者的疾病分期（P = 0.041）与CD1表达之间存在显著相关性。性别（P = 0.055）、年龄（P = 0.256）、吸烟和药物滥用史（P = 0.192）、肿瘤位置（P = 0.90）、疾病分级（P = 0.515）和地理区域（P = 0.466）与喉鳞状细胞癌患者的CD1表达之间均无显著相关性。本研究结果显示，与其他研究相比，喉鳞状细胞癌患者中CD1的表达更高（91.5%）。根据结果我们可以得出结论，疾病分期可显著影响鳞状细胞癌患者的CD1表达。
