Zhang Jun, Liu Yi, Yu Chang-Jun, Dai Fu, Xiong Jie, Li Hong-Jun, Wu Zheng-Sheng, Ding Rui, Wang Hong
Department of General Surgery, Third Affiliated Hospital (Hefei First People's Hospital) of Anhui Medical University, Hefei, China.
Department of Gastrointestinal Surgery, First Affiliated Hospital of Anhui Medical University, Hefei, China.
Mediators Inflamm. 2017;2017:5432818. doi: 10.1155/2017/5432818. Epub 2017 Jun 13.
Gastric cancer continues to be the second most frequent cause of cancer deaths worldwide. However, the exact molecular mechanisms are still unclear. Further research to find potential targets for therapy is critical and urgent. In this study, we found that ARPC2 promoted cell proliferation and invasion in the human cancer cell line MKN-28 using a cell total number assay, MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay, cell colony formation assay, migration assay, invasion assay, and wound healing assay. For downstream pathways, CTNND1, EZH2, BCL2L2, CDH2, VIM, and EGFR were upregulated by ARPC2, whereas PTEN, BAK, and CDH1 were downregulated by ARPC2. In a clinical study, we examined the expression of ARPC2 in 110 cases of normal human gastric tissues and 110 cases of human gastric cancer tissues. ARPC2 showed higher expression in gastric cancer tissues than in normal gastric tissues. In the association analysis of 110 gastric cancer tissues, ARPC2 showed significant associations with large tumor size, lymph node invasion, and high tumor stage. In addition, ARPC2-positive patients exhibited lower RFS and OS rates compared with ARPC2-negative patients. We thus identify that ARPC2 plays an aneretic role in human gastric cancer and provided a new target for gastric cancer therapy.
胃癌仍然是全球第二大常见癌症死亡原因。然而，确切的分子机制仍不清楚。进一步研究寻找潜在的治疗靶点至关重要且紧迫。在本研究中，我们使用细胞总数测定法、MTT（3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2-H-四氮唑溴盐）测定法、细胞集落形成测定法、迁移测定法、侵袭测定法和伤口愈合测定法，发现ARPC2在人癌细胞系MKN-28中促进细胞增殖和侵袭。对于下游通路，ARPC2上调CTNND1、EZH2、BCL2L2、CDH2、VIM和EGFR，而ARPC2下调PTEN、BAK和CDH1。在一项临床研究中，我们检测了110例正常人胃组织和110例人胃癌组织中ARPC2的表达。ARPC2在胃癌组织中的表达高于正常胃组织。在对110例胃癌组织的关联分析中，ARPC2与肿瘤体积大、淋巴结侵袭和肿瘤分期高显著相关。此外，与ARPC2阴性患者相比，ARPC2阳性患者的无复发生存率（RFS）和总生存率（OS）较低。因此，我们确定ARPC2在人类胃癌中起促癌作用，并为胃癌治疗提供了一个新靶点。
