Futoma-Kołoch Bożena, Dudek Bartłomiej, Kapczyńska Katarzyna, Krzyżewska Eva, Wańczyk Martyna, Korzekwa Kamila, Rybka Jacek, Klausa Elżbieta, Bugla-Płoskońska Gabriela
Department of Microbiology, Institute of Genetics and Microbiology, University of Wrocław, 51-148 Wrocław, Poland.
Department of Immunology of Infectious Diseases, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wrocław, Poland.
Int J Mol Sci. 2017 Jul 11;18(7):1459. doi: 10.3390/ijms18071459.
A new emerging phenomenon is the association between the incorrect use of biocides in the process of disinfection in farms and the emergence of cross-resistance in populations. Adaptation of the microorganisms to the sub-inhibitory concentrations of the disinfectants is not clear, but may result in an increase of sensitivity or resistance to antibiotics, depending on the biocide used and the challenged serovar. Exposure of five subsp. serovar Senftenberg ( Senftenberg) strains to triamine-containing disinfectant did not result in variants with resistance to antibiotics, but has changed their susceptibility to normal human serum (NHS). Three biocide variants developed reduced sensitivity to NHS in comparison to the sensitive parental strains, while two isolates lost their resistance to serum. For Senftenberg, which exhibited the highest triamine tolerance (6 × MIC) and intrinsic sensitivity to 22.5% and 45% NHS, a downregulation of flagellin and enolase has been demonstrated, which might suggest a lower adhesion and virulence of the bacteria. This is the first report demonstrating the influence of biocide tolerance on NHS resistance. In conclusion, there was a potential in Senftenberg to adjust to the conditions, where the biocide containing triamine was present. However, the adaptation did not result in the increase of antibiotic resistance, but manifested in changes within outer membrane proteins' patterns. The strategy of bacterial membrane proteins' analysis provides an opportunity to adjust the ways of infection treatments, especially when it is connected to the life-threating bacteremia caused by species.
一种新出现的现象是，农场消毒过程中生物杀灭剂的不当使用与种群中交叉耐药性的出现之间存在关联。微生物对消毒剂亚抑制浓度的适应性尚不清楚，但可能会导致对抗生素的敏感性增加或耐药性增强，这取决于所使用的生物杀灭剂和所挑战的血清型。将5株肠炎沙门氏菌亚种森夫滕贝格（Senftenberg）菌株暴露于含三胺的消毒剂中，并未产生对抗生素耐药的变体，但改变了它们对正常人血清（NHS）的敏感性。与敏感亲本菌株相比，三种生物杀灭剂变体对NHS的敏感性降低，而两株分离株失去了对血清的耐药性。对于表现出最高三胺耐受性（6×MIC）且对22.5%和45%NHS具有固有敏感性的森夫滕贝格菌株，已证明鞭毛蛋白和烯醇酶的表达下调，这可能表明该细菌的黏附性和毒力较低。这是第一份证明生物杀灭剂耐受性对NHS耐药性影响的报告。总之，森夫滕贝格菌株有潜力适应存在含三胺生物杀灭剂的条件。然而，这种适应并未导致抗生素耐药性增加，而是表现为外膜蛋白模式的变化。细菌膜蛋白分析策略为调整感染治疗方式提供了机会，尤其是在涉及由该菌种引起的危及生命的菌血症时。
