Horváthová Mira, Ilavská Silvia, Štefíková Kornélia, Szabová Michaela, Krivošíková Zora, Jahnová Eva, Tulinská Jana, Spustová Viera, Gajdoš Martin
Department of Immunology and Immunotoxicology, Faculty of Medicine, Slovak Medical University, 833 03 Bratislava, Slovakia.
Department of Clinical and Experimental Pharmacology, Faculty of Medicine, Slovak Medical University, 83303 Bratislava, Slovakia.
Int J Environ Res Public Health. 2017 Jul 11;14(7):751. doi: 10.3390/ijerph14070751.
The age-related changes and hormonal deprivation in postmenopausal women are associated with the immune response alteration. The excessive fat accumulation, local and systemic inflammation may lead to dysregulation in immune function and relevant health problems, including obesity and osteoporosis. We analyzed the expression of cell surface markers in the venous blood specimens, stained with fluorophores-conjugated monoclonal antibodies and analysed by multicolour flow cytometry. The significant changes of cytotoxic, naive, and memory T-lymphocytes, plasmacytoid dendritic cells (DCs) were in postmenopausal women versus fertile women. Body mass index (BMI) affected markedly the cell surface expression of CD265/RANK. Osteoporosis is linked to reduced percentage of plasmacytoid DCs, and elevated natural Treg cells ( < 0.05). The confounding factors such as women age, BMI, bone mineral density (BMD), waist size and tissue fat affect the expression of RANK on myeloid DCs and CD40L on T-lymphocytes that might be the immunophenotypic modulators after menopause.
绝经后女性与年龄相关的变化和激素缺乏与免疫反应改变有关。过多的脂肪堆积、局部和全身炎症可能导致免疫功能失调及相关健康问题，包括肥胖和骨质疏松。我们分析了静脉血标本中细胞表面标志物的表达，这些标本用荧光团偶联的单克隆抗体染色，并通过多色流式细胞术进行分析。与育龄女性相比，绝经后女性的细胞毒性、初始和记忆性T淋巴细胞、浆细胞样树突状细胞（DC）有显著变化。体重指数（BMI）显著影响CD265/RANK的细胞表面表达。骨质疏松与浆细胞样DC百分比降低和自然调节性T细胞升高有关（<0.05）。女性年龄、BMI、骨矿物质密度（BMD）、腰围和组织脂肪等混杂因素会影响髓样DC上RANK的表达以及T淋巴细胞上CD40L的表达，这些可能是绝经后的免疫表型调节因子。
