Gao Li, Li Ye, Yang Yong-Jie, Zhang Dong-Yan
College of Sports and Health, Shandong Sport University, Jinan, China.
Front Physiol. 2021 Oct 29;12:729910. doi: 10.3389/fphys.2021.729910. eCollection 2021.
Using RNA-sequencing technology to screen the effect of moderate-intensity treadmill exercise on the sensitive genes that affect bone mass in the peripheral blood mononuclear cells (PBMCs) of ovariectomized (OVX) rats. Three-month-old female Sprague-Dawley rats of Specific Pathogen Free (SPF) grade were randomly divided into the sham operation (SHAM) group, OVX group, and OVX combined exercise (OVX + EX) group. The OVX + EX group performed moderate-intensity treadmill exercise for 17 weeks. Then, the body composition and bone mineral density (BMD) were measured, and the bone microstructure of the femur was observed. PBMCs were collected from the abdominal aorta, and the differential genes were analyzed by transcriptome sequencing to further screen sensitive genes. (1) In the OVX group, the body weight and body fat content were significantly higher than in the SHAM group while the muscle content and BMD were significantly lower than the SHAM group. (2) The trabecular bone parameters in the OVX group were significantly lower than in the SHAM group, and they were significantly higher in the OVX + EX group than in the OVX group. When compared with the SHAM group, the microstructure of the distal femur trabecular in the OVX group was severely damaged, suggest that the morphological structure of trabecular bone is severely damaged, the number of trabecular bones is reduced, and the thickness becomes thinner, which lead to the widening of the trabecular bone space and the appearance of osteoporosis. The number and continuity of the trabecular bones were higher in the OVX + EX group than in the OVX group. (3) A Venn diagram showed that there were 58 common differential genes, and the differential genes were mainly enriched in the PI3K-Akt signaling pathway. Five sensitive genes were screened including CCL2, Nos3, Tgfb3, ITGb4, and LpL. The expression of CCL2, Nos3, and Tgfb3 genes was closely related to multiple bone parameters. Moderate-intensity treadmill exercise may improve the body composition and bone mass of the OVX group by upregulating CCL2 and other genes of the PBMC. The PBMCs in the peripheral blood can be a useful tool for monitoring the effect of exercise on bone health in postmenopausal osteoporosis.
运用RNA测序技术筛选中等强度跑步机运动对去卵巢（OVX）大鼠外周血单个核细胞（PBMC）中影响骨量的敏感基因的作用。将3月龄特定病原体-free（SPF）级雌性Sprague-Dawley大鼠随机分为假手术（SHAM）组、OVX组和OVX联合运动（OVX + EX）组。OVX + EX组进行17周的中等强度跑步机运动。然后，测量身体成分和骨密度（BMD），并观察股骨的骨微结构。从腹主动脉采集PBMC，通过转录组测序分析差异基因以进一步筛选敏感基因。（1）在OVX组中，体重和体脂含量显著高于SHAM组，而肌肉含量和BMD显著低于SHAM组。（2）OVX组的小梁骨参数显著低于SHAM组，且OVX + EX组显著高于OVX组。与SHAM组相比，OVX组股骨远端小梁的微结构严重受损，表明小梁骨的形态结构严重受损，小梁骨数量减少，厚度变薄，导致小梁骨间隙增宽并出现骨质疏松。OVX + EX组的小梁骨数量和连续性高于OVX组。（3）维恩图显示有58个共同差异基因，这些差异基因主要富集于PI3K-Akt信号通路。筛选出5个敏感基因，包括CCL2、Nos3、Tgfb3、ITGb4和LpL。CCL2、Nos3和Tgfb3基因的表达与多个骨参数密切相关。中等强度跑步机运动可能通过上调PBMC的CCL2等基因改善OVX组的身体成分和骨量。外周血中的PBMC可作为监测运动对绝经后骨质疏松症患者骨骼健康影响的有用工具。
