Rinkel Jan, Rabe Patrick, Chen Xinlu, Köllner Tobias G, Chen Feng, Dickschat Jeroen S
Kekulé-Institute of Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Straße 1, 53121, Bonn, Germany.
Department of Plant Sciences, University of Tennessee, 2431 Joe Johnson Drive, Knoxville, TN, 37996-4561, USA.
Chemistry. 2017 Aug 4;23(44):10501-10505. doi: 10.1002/chem.201702704. Epub 2017 Jul 11.
Two diterpene cyclases, one from the social amoeba Dictyostelium discoideum and the other from the bacterium Streptomyces clavuligerus, with products containing a Z-configured double bond between the original C2 and C3 of geranylgeranyl diphosphate, were extensively investigated for their mechanisms through isotopic labelling experiments. The participation of geranyllinalyl diphosphate, in analogy to the role of linalyl and nerolidyl diphosphate for mono- and sesquiterpene biosynthesis, as an intermediate towards diterpenes with a Z-configured C2=C3 double bond is discussed.
通过同位素标记实验，对两种二萜环化酶进行了广泛的机制研究，其中一种来自社会性变形虫盘基网柄菌，另一种来自细菌克拉维链霉菌，它们的产物在香叶基香叶基二磷酸的原始C2和C3之间含有一个Z构型的双键。本文讨论了香叶基芳樟基二磷酸作为具有Z构型C2 = C3双键的二萜生物合成中间体的参与情况，这类似于芳樟基二磷酸和橙花叔基二磷酸在单萜和倍半萜生物合成中的作用。
