Animal Science and Health Cluster, Institute of Biology Leiden, Leiden University, The Netherlands; Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan.
Animal Science and Health Cluster, Institute of Biology Leiden, Leiden University, The Netherlands.
Reprod Toxicol. 2017 Oct;73:292-311. doi: 10.1016/j.reprotox.2017.07.002. Epub 2017 Jul 8.
The ability to culture complex organs is currently an important goal in biomedical research. It is possible to grow organoids (3D organ-like structures) in vitro; however, a major limitation of organoids, and other 3D culture systems, is the lack of a vascular network. Protocols developed for establishing in vitro vascular networks typically use human or rodent cells. A major technical challenge is the culture of functional (perfused) networks. In this rapidly advancing field, some microfluidic devices are now getting close to the goal of an artificially perfused vascular network. Another development is the emergence of the zebrafish as a complementary model to mammals. In this review, we discuss the culture of endothelial cells and vascular networks from mammalian cells, and examine the prospects for using zebrafish cells for this objective. We also look into the future and consider how vascular networks in vitro might be successfully perfused using microfluidic technology.
目前，培养复杂器官的能力是生物医学研究的一个重要目标。在体外培养类器官（3D 类器官样结构）是可能的；然而，类器官和其他 3D 培养系统的一个主要限制是缺乏血管网络。为建立体外血管网络而开发的方案通常使用人类或啮齿动物细胞。一个主要的技术挑战是培养功能性（灌注）网络。在这个快速发展的领域中，一些微流控设备现在已经接近人工灌注血管网络的目标。另一个发展是斑马鱼作为哺乳动物的补充模型的出现。在这篇综述中，我们讨论了从哺乳动物细胞培养内皮细胞和血管网络，并探讨了使用斑马鱼细胞实现这一目标的前景。我们还展望未来，考虑如何使用微流控技术成功地对体外血管网络进行灌注。
