Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224007, Jiangsu, People's Republic of China.
Research Institute of Applied Biology, Shanxi University, Taiyuan 030006, Shanxi, People's Republic of China.
Int J Biol Macromol. 2017 Dec;105(Pt 1):219-227. doi: 10.1016/j.ijbiomac.2017.07.033. Epub 2017 Jul 8.
The mitochondrial genome (mitogenome) can provide information for phylogenetic analyses and evolutionary biology. We sequenced, annotated, and characterized the mitogenome of Euproctis similis. The complete mitogenome is 15,437bp in length, containing 13 protein-coding genes (PCGs), 22 transfer RNA genes, two ribosomal RNA genes, and a control region (A+T-rich region). The A+T content in the mitogenome was 80.16%. All PCGs use standard ATN as a start codon, with the exception of cytochrome c coxidase 1 (cox1) with CGA. A gene rearrangement (trnM) was found. All transfer RNA (tRNA) genes have a typical clover-leaf structure except for trnS1 (AGN). Phylogenetic analysis was performed using Bayesian Inference and Maximum Likelihood based on the amino acid and nucleotide sequences of 13 mitochondrial PCGs. The well-supported phylogenetic relationships can be generally described as: Notodontidae+(Erebidae+(Nolidae+(Euteliidae+Noctuidae))). The tree support that E. similis shares a close ancestry with Erebidae insects. Our results indicate that Erebidae is a sister group to the other families (Euteliidae, Nolidae, and Noctuidae).
线粒体基因组（mitogenome）可以为系统发育分析和进化生物学提供信息。我们对斜纹夜蛾（Euproctis similis）的线粒体基因组进行了测序、注释和特征分析。完整的线粒体基因组长 15437bp，包含 13 个蛋白质编码基因（PCGs）、22 个转移 RNA 基因、两个核糖体 RNA 基因和一个控制区（富含 A+T 区）。线粒体基因组的 A+T 含量为 80.16%。所有 PCGs 使用标准的 ATN 作为起始密码子，除了细胞色素 c 氧化酶 1（cox1）使用 CGA。发现了一个基因重排（trnM）。除了 trnS1（AGN）外，所有转移 RNA（tRNA）基因都具有典型的三叶草结构。基于 13 个线粒体 PCGs 的氨基酸和核苷酸序列，采用贝叶斯推断和最大似然法进行了系统发育分析。支持良好的系统发育关系可以大致描述为：Notodontidae+（Erebidae+（Nolidae+（Euteliidae+Noctuidae）））。该树支持斜纹夜蛾与夜蛾科昆虫具有密切的亲缘关系。我们的结果表明，夜蛾科是与其他科（Euteliidae、Nolidae 和 Noctuidae）的姐妹群。
