College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, Henan 466000, China.
Yongqiao District Plant Protection Station, Suzhou, Anhui 234000, China.
Int J Biol Macromol. 2019 Feb 15;123:485-493. doi: 10.1016/j.ijbiomac.2018.10.149. Epub 2018 Oct 22.
The complete mitochondrial genome (mitogenome) of an important medicinal insect Hydrillodes repugnalis (Lepidoptera: Noctuoidea) was sequenced and analyzed. The mitogenome is circular with 15,570 bp long, and shows typical gene content and arrangement. Nucleotide composition is highly biased toward A + T nucleotides (81.1%). All protein-coding genes (PCGs) initiate with canonical start codon ATN, except for cox1 being CGA. The typical stop codon TAA is used for most PCGs, while the nad4l uses the TAG, and cox1 and cox2 use incomplete termination codon T. All tRNAs have a typical clover-leaf structure, except for trnS1 (AGN) lacking the dihydrouridine arm. Comparative mitogenome analysis showed that the motif "ATGATAA" between atp8 and atp6, and the motif "ATACTAA" between trnS2 and nad1 are commonly present in noctuoid mitogenomes. In A + T-rich regions, the motif "ATAGA" and subsequent poly-T structure, the motif "ATTTA" and followed macrosatellite (AT) element and an "A"-rich 3' end upstream of the trnM gene can be recognized across noctuoid mitogenomes. Phylogenetic analyses showed that H. repugnalis is nested into the Erebidae clade, consistently being sister to the Aganainae. Within Noctuoidea, the (Notodontidae + (Erebidae + (Nolidae + (Euteliidae + Noctuidae)))) was consistently recovered firstly based on multiple mitochondrial datasets.
已测序并分析了一种重要药用昆虫 Hydrillodes repugnalis（鳞翅目：夜蛾总科）的完整线粒体基因组（线粒体基因组）。线粒体基因组是圆形的，长 15570bp，显示出典型的基因含量和排列。核苷酸组成高度偏向 A+T 核苷酸（81.1%）。除 cox1 为 CGA 外，所有蛋白质编码基因（PCG）均以典型的起始密码子 ATN 起始。典型的终止密码子 TAA 用于大多数 PCG，而 nad4l 使用 TAG，cox1 和 cox2 使用不完全终止密码子 T。所有 tRNA 都具有典型的三叶形结构，除了 trnS1（AGN）缺少二氢尿嘧啶臂。比较线粒体基因组分析表明，atp8 和 atp6 之间的“ATGATAA”和 trnS2 和 nad1 之间的“ATACTAA” motif 普遍存在于夜蛾线粒体基因组中。在 A+T 丰富的区域，可以识别到“ATAGA”和随后的多 T 结构、“ATTTA”和随后的宏卫星（AT）元件以及 trnM 基因上游的“A”-丰富 3'端，这些特征普遍存在于夜蛾线粒体基因组中。系统发育分析表明，H. repugnalis 嵌套在 Erebidae 分支中，始终与 Aganainae 为姐妹关系。在 Noctuoidea 中，基于多个线粒体数据集，首先一致恢复了（Notodontidae+（Erebidae+（Nolidae+（Euteliidae+Noctuidae）））。
