Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia.
Faculty of Chemistry, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia.
Biomolecules. 2019 Aug 21;9(9):391. doi: 10.3390/biom9090391.
The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different 'Oblačinska' sour cherry clones and to assess the chemical fingerprint of this cultivar. Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system. Glucose was the most abundant sugar, followed by fructose and sucrose. Some samples had high level of stress sugars, especially trehalose. Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and -coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after. According to the principal component analysis (PCA), fructose, maltose, maltotriose, sorbitol, and trehalose were the most important sugars in separating pollen samples. PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles. Large differences in chemical composition of studied 'Oblačinska sour cherry' clone pollen were shown, proving that it is not a cultivar, but population. Finally, due to the highest level of phenolics, clones IV/8, XV/3, and VIII/1 could be singled out as a promising one for producing functional food and/or in medicinal treatments.
本研究旨在分析从 15 个不同的‘Oblačinska’酸樱桃品种的花粉中提取的糖和酚类物质，并评估该品种的化学指纹图谱。使用高效阴离子交换色谱（HPAEC）与脉冲安培检测（PAD）进行碳水化合物分析，而多酚则通过超高效液相色谱-二极管阵列检测器-串联质谱（UHPLC-DAD-MS/MS）系统进行分析。葡萄糖是最丰富的糖，其次是果糖和蔗糖。一些样品含有高水平的应激糖，特别是海藻糖。芦丁是数量最多的多酚，高达 181.12 毫克/千克（III/9 号克隆），其后是绿原酸（高达 59.93 毫克/千克，在 III/9 号克隆中）和 -香豆酸（高达 53.99 毫克/千克，在 VIII/1 号克隆中）。根据主成分分析（PCA），果糖、麦芽糖、麦芽三糖、山梨糖醇和海藻糖是分离花粉样品的最重要的糖。PCA 显示，根据酚类物质的含量和不同的图谱，将 VIII/1、IV/8、III/9 和 V/P 号克隆分离出来。研究表明，‘Oblačinska’酸樱桃品种花粉的化学成分存在较大差异，证明其不是一个品种，而是一个群体。最后，由于酚类物质含量最高，IV/8、XV/3 和 VIII/1 号克隆可以被挑选出来，作为生产功能性食品和/或药用治疗的有前途的品种。
