BioMedicine Discovery Institute & Department of Physiology, Monash University, Clayton, VIC, Australia.
J Neuroendocrinol. 2017 Oct;29(10). doi: 10.1111/jne.12504.
Diet-induced obesity is associated with hypothalamic inflammation and this phenomenon has been proposed to explain leptin resistance. In the present study, we used a short-term high-fat diet (HFD) paradigm for 10 days and analysed the cellular and physiological responses to leptin administration in C57BL6 mice. In parallel, we performed glial fibrillary acidic protein immunostaining to measure the presence of astrocytes in the arcuate nucleus of the hypothalamus (ARH) after 10 days and 20 weeks of HFD. Interestingly, the results obtained demonstrate that the presence of star-like astrocytes is significantly increased after 10 days of HFD, although this is not associated with the absence of cellular and physiological response to leptin administration in mice. Taken together, the results of the present study suggest that star-like astrocytes rapidly increase in numbers in the ARH in response to HFD, although this phenomenon cannot explain the development of leptin resistance by itself.
饮食诱导的肥胖与下丘脑炎症有关，这种现象被认为可以解释瘦素抵抗。在本研究中，我们使用了为期 10 天的短期高脂肪饮食（HFD）范式，并分析了 C57BL6 小鼠对瘦素给药的细胞和生理反应。平行地，我们进行了神经胶质纤维酸性蛋白免疫染色，以测量在 HFD 10 天和 20 周后下丘脑弓状核（ARH）中星形胶质细胞的存在。有趣的是，获得的结果表明，在 HFD 10 天后，星形细胞的存在显著增加，尽管这与小鼠对瘦素给药的细胞和生理反应缺失无关。总之，本研究的结果表明，星形细胞在 ARH 中的数量在 HFD 作用下迅速增加，尽管这一现象本身并不能解释瘦素抵抗的发展。
