Souza Vilela Podestá Tatiane, Venzel Rosembach Tiago, Aparecida Dos Santos Anésia, Lobato Martins Marcelo
Departamento de Física, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
PLoS One. 2017 Jul 10;12(7):e0180777. doi: 10.1371/journal.pone.0180777. eCollection 2017.
In multicellular organisms, cell motility is central in all morphogenetic processes, tissue maintenance, wound healing and immune surveillance. Hence, the control of cell motion is a major demand in the creation of artificial tissues and organs. Here, cell migration assays on plastic 2D surfaces involving normal (MDCK) and tumoral (B16F10) epithelial cell lines were performed varying the initial density of plated cells. Through time-lapse microscopy quantities such as speed distributions, velocity autocorrelations and spatial correlations, as well as the scaling of mean-squared displacements were determined. We find that these cells exhibit anomalous diffusion with q-Weibull speed distributions that evolves non-monotonically to a Maxwellian distribution as the initial density of plated cells increases. Although short-ranged spatial velocity correlations mark the formation of small cell clusters, the emergence of collective motion was not observed. Finally, simulational results from a correlated random walk and the Vicsek model of collective dynamics evidence that fluctuations in cell velocity orientations are sufficient to produce q-Weibull speed distributions seen in our migration assays.
在多细胞生物中，细胞运动性在所有形态发生过程、组织维持、伤口愈合和免疫监视中都起着核心作用。因此，控制细胞运动是制造人工组织和器官的一项主要要求。在这里，我们在塑料二维表面上对正常（MDCK）和肿瘤（B16F10）上皮细胞系进行了细胞迁移实验，改变接种细胞的初始密度。通过延时显微镜测定了诸如速度分布、速度自相关和空间相关性等数量，以及均方位移的标度。我们发现，这些细胞表现出具有q-韦布尔速度分布的反常扩散，随着接种细胞的初始密度增加，这种分布非单调地演变为麦克斯韦分布。尽管短程空间速度相关性标志着小细胞簇的形成，但未观察到集体运动的出现。最后，相关随机游走和集体动力学的维塞克模型的模拟结果表明，细胞速度方向的波动足以产生我们在迁移实验中看到的q-韦布尔速度分布。
