Smith-Vaughan Heidi C, Beissbarth Jemima, Bowman Jacinta, Hare Kim M, Price Erin P, Pickering Janessa, Lehmann Deborah, Chang Anne B, Morris Peter S, Marsh Robyn L, Leach Amanda J
Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory Australia.
School of Medicine, Griffith University, Gold Coast, Queensland Australia.
Pneumonia (Nathan). 2016 Aug 18;8:13. doi: 10.1186/s41479-016-0013-y. eCollection 2016.
Non-typeable (NTHi)-associated ear and respiratory diseases (including pneumonia) represent a major health burden in many parts of the world. NTHi strains retrieved from the upper airways commonly reflect those found in the lower airways. Despite growing genomic and genotyping data on NTHi, there remains a limited understanding of global and regional NTHi population structures. The aim of this study was to determine whether nasopharyngeal carriage in four Australian paediatric groups at varying risk of NTHi colonisation was dominated by the same NTHi genotypes. Genotyping data generated by PCR-ribotyping were evaluated for 3070 NTHi isolates colonising the nasopharynges of Aboriginal and non-Aboriginal children enrolled in four longitudinal studies in three separate urban and remote regions of Australia. Several NTHi PCR-ribotypes dominated in nasopharyngeal carriage, irrespective of study setting. Principal coordinates analysis confirmed a cluster of common PCR-ribotypes among all cohorts. In conclusion, we identified dominant PCR-ribotypes common to geographically disparate Australian paediatric populations. Future genomic analyses will shed further light on the precise factors underlying the dominance of certain NTHi strains in nasopharyngeal carriage.
不可分型（NTHi）相关的耳部和呼吸道疾病（包括肺炎）在世界许多地区构成了重大的健康负担。从上呼吸道分离出的NTHi菌株通常反映了在下呼吸道发现的菌株。尽管关于NTHi的基因组和基因分型数据不断增加，但对全球和区域NTHi种群结构的了解仍然有限。本研究的目的是确定在四个澳大利亚儿科群体中，具有不同NTHi定植风险的鼻咽部携带是否由相同的NTHi基因型主导。对在澳大利亚三个不同城市和偏远地区的四项纵向研究中招募的原住民和非原住民儿童鼻咽部定植的3070株NTHi分离株，评估了通过PCR核糖体分型产生的基因分型数据。无论研究环境如何，几种NTHi PCR核糖体分型在鼻咽部携带中占主导地位。主坐标分析证实了所有队列中存在一组常见的PCR核糖体分型。总之，我们确定了地理上不同的澳大利亚儿科人群中常见的主导PCR核糖体分型。未来的基因组分析将进一步揭示某些NTHi菌株在鼻咽部携带中占主导地位的精确因素。
