Lavis Luke D
Howard Hughes Medical Institute , Janelia Research Campus, 19700 Helix Drive, Ashburn, Virginia 20147, United States.
Biochemistry. 2017 Oct 3;56(39):5165-5170. doi: 10.1021/acs.biochem.7b00529. Epub 2017 Aug 9.
Chemistry, once king of fluorescence microscopy, was usurped by the field of fluorescent proteins. The increased demands of modern microscopy techniques on the "photon budget" require better and brighter fluorophores, causing a renewed interest in synthetic dyes. Here, we review the recent advances in biochemistry, protein engineering, and organic synthesis that have allowed a triumphant return of chemical fluorophores to modern biological imaging.
化学曾是荧光显微镜领域的王者，但如今已被荧光蛋白领域取而代之。现代显微镜技术对“光子预算”的要求不断提高，这就需要更好、更亮的荧光团，从而引发了对合成染料的新兴趣。在此，我们综述了生物化学、蛋白质工程和有机合成方面的最新进展，这些进展使得化学荧光团得以成功回归现代生物成像领域。
