Krakowka S, Ringler S S
Vet Immunol Immunopathol. 1986 Mar;11(3):281-9. doi: 10.1016/0165-2427(86)90007-3.
Six day in vitro cultures of canine mononuclear leukocytes with or without prior carbonyl iron depletion were established with 5 commonly employed mitogens. Cultures were assessed for B-cell differentiation by induction of cytoplasmic and supernatant released IgG, M and A and T-cell differentiation by expression of Thy-1 antigen on the cell surface of lymphoblasts. The mitogen concanavalin A was shown to be a restricted T-cell mitogen, whereas pokeweed mitogen and protein A from Staphylococcus aureus stimulated maximal B-cell differentiation. These data establish the mitogenic specificity for canine lymphocytes in unfractionated peripheral blood leukocyte cultures and will permit an in vitro evaluation of various substances upon T- and B-lymphocyte functions.
使用5种常用的有丝分裂原建立了犬单核白细胞的6天体外培养物，这些培养物有的经过羰基铁预先耗尽处理，有的没有。通过诱导细胞质和上清液中释放的IgG、M和A来评估B细胞分化，并通过淋巴母细胞表面Thy-1抗原的表达来评估T细胞分化。已证明有丝分裂原刀豆球蛋白A是一种受限的T细胞有丝分裂原，而商陆有丝分裂原和金黄色葡萄球菌蛋白A能刺激最大程度的B细胞分化。这些数据确立了未分级外周血白细胞培养物中犬淋巴细胞的有丝分裂特异性，并将允许对各种物质对T和B淋巴细胞功能的体外评估。
