使用溴脱氧尿苷掺入法或中期筛选对丝裂原诱导的增殖淋巴细胞进行免疫标记分析。葡萄球菌蛋白A是CD4 +淋巴细胞的有效丝裂原。

Immunological marker analysis of mitogen-induced proliferating lymphocytes using BrdU incorporation or screening of metaphases. Staphylococcal protein A is a potent mitogen for CD4+ lymphocytes.

作者信息

Adriaansen H J, Osman C, van Dongen J J, Wijdenes-de Bresser J H, Kappetijn-van Tilborg C M, Hooijkaas H

机构信息

Department of Immunology, University Hospital Dijkzigt, Rotterdam, The Netherlands.

出版信息

Scand J Immunol. 1990 Dec;32(6):687-94. doi: 10.1111/j.1365-3083.1990.tb03211.x.

DOI:10.1111/j.1365-3083.1990.tb03211.x

PMID:1980157

Abstract

The proliferative effects of the mitogens phytohaemagglutinin (PHA), concanavalin A (Con A), pokeweed mitogen (PWM), and staphylococcal protein A (SpA) were investigated using two different methods which enable immunological marker analysis of proliferating cells: either surface marker labelling followed by BrdU incorporation or screening of metaphases after surface marker labelling. Therefore peripheral blood mononuclear cells from six healthy volunteers were stimulated with these four mitogens. Both PHA and Con A gave rise to more CD8+ than CD4+ proliferating cells. PHA, but not Con A, induced B-cell proliferation as well. PWM mainly caused T-cell proliferation. SpA also appeared to be a potent T-cell mitogen in addition to its capacity to induce B-cell proliferation. However, in contrast to the other mitogens SpA predominantly stimulated CD4+ cells.

摘要

使用两种不同方法研究了有丝分裂原植物血凝素（PHA）、刀豆球蛋白A（Con A）、商陆有丝分裂原（PWM）和葡萄球菌蛋白A（SpA）的增殖作用，这两种方法能够对增殖细胞进行免疫标记分析：要么先进行表面标记再掺入溴脱氧尿苷（BrdU），要么在表面标记后筛选中期细胞。因此，用这四种有丝分裂原刺激了六名健康志愿者的外周血单个核细胞。PHA和Con A诱导产生的增殖细胞中CD8+比CD4+更多。PHA还诱导了B细胞增殖，而Con A没有。PWM主要引起T细胞增殖。SpA除了具有诱导B细胞增殖的能力外，似乎还是一种有效的T细胞有丝分裂原。然而，与其他有丝分裂原不同，SpA主要刺激CD4+细胞。