长链非编码RNA linc00346促进膀胱癌的恶性表型。
Long noncoding RNA linc00346 promotes the malignant phenotypes of bladder cancer.
作者信息
Ye Tingyu, Ding Wei, Wang Nanxiong, Huang Hang, Pan Yue, Wei Anyang
机构信息
Department of Urology, Nanfang Hospital of Southern Medical University, Guangzhou, China; Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Department of Urology, Nanfang Hospital of Southern Medical University, Guangzhou, China.
出版信息
Biochem Biophys Res Commun. 2017 Sep 9;491(1):79-84. doi: 10.1016/j.bbrc.2017.07.045. Epub 2017 Jul 11.
BACKGROUND
More and more reports have demonstrated that long noncoding RNAs (lncRNAs) play an important role in the development of a variety of carcinomas, including bladder cancer. However, only a small fraction of them have been characterized. Linc00346 have been found to be upregulated in bladder cancer tissues compared to normal tissues in a microarray-based lncRNA profiling study. In this study, we would like to explore the expression pattern and functional role of linc00346 in bladder cancer.
METHODS
We determined the expression of linc00346 in a cohort of bladder cancer tissues with matched normal tissues as well as human bladder cancer cell lines. We investigated the biological function of linc00346 with CCK-8 assay, colony formation assay, flow cytometry analysis, transwell assay and tumor xenografts mice model.
RESULTS
We found that linc00346 was upregulated in bladder cancer tissues compared to normal tissues. Knockdown of linc00346 inhibited bladder cancer cell proliferation and migration, induced cell cycle arrest and cell apoptosis.
CONCLUSION
Our study demonstrates that linc00346 could be a potential oncogene and a therapeutic target in bladder cancer.
背景
越来越多的报道表明，长链非编码RNA（lncRNAs）在包括膀胱癌在内的多种癌症的发生发展中发挥着重要作用。然而，其中只有一小部分得到了表征。在一项基于微阵列的lncRNA谱分析研究中，发现与正常组织相比，Linc00346在膀胱癌组织中上调。在本研究中，我们旨在探讨Linc00346在膀胱癌中的表达模式及功能作用。
方法
我们检测了一组配对的膀胱癌组织及正常组织以及人膀胱癌细胞系中Linc00346的表达。我们通过CCK-8检测、集落形成检测、流式细胞术分析、Transwell检测及肿瘤异种移植小鼠模型研究了Linc00346的生物学功能。
结果
我们发现与正常组织相比，Linc00346在膀胱癌组织中上调。敲低Linc00346可抑制膀胱癌细胞的增殖和迁移，诱导细胞周期停滞及细胞凋亡。
结论
我们的研究表明，Linc00346可能是膀胱癌中的一个潜在癌基因及治疗靶点。
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