Asari Mohd Asnizam, Sirajudeen K N S, Mohd Yusof Nurul Aiman, Mohd Amin Mohamad Syabil Ikhwan
Department of Anatomy, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia, Bandar Indera Mahkota, Kuantan, Pahang, Malaysia.
J Tradit Complement Med. 2021 Oct 12;12(4):361-366. doi: 10.1016/j.jtcme.2021.10.001. eCollection 2022 Jul.
Exposure to chronic stress induces oxidative damage which alters the dynamic balance between antioxidant and pro-oxidant activities in the brain. Tualang honey (TH) is a Malaysian wild multifloral honey which has been shown to contain high amount antioxidants. DHA-rich fish oil is a form of omega-3 fatty acids found in fish which also possesses some antioxidant activity. This study aimed to evaluate anti-stress activity of DHA-rich fish oil, TH and their combination on several parameters of oxidative stress in chronic stress rat model.
Fifty male Sprague Dawley rats were divided into (i) control, (ii) stress-exposed, (iii) stress-exposed and treated with TH (1 g/kg body weight twice daily), (iv) stress-exposed and treated with DHA-rich fish oil (450 mg/kg body weight twice daily), and (v) stress-exposed and treated with a combination of TH and DHA-rich fish oil. The chronic stress regimen consisted of a combination of restraint stress and a swim stress test for 28 days.
DHA-rich fish oil and TH significantly (p < 0.05) supressed stress-induced elevation of serum corticosterone and lipid peroxidation, and caused a significant increase in total antioxidant capacity. For glutathione status, only TH significantly reduced stress-induced elevation of oxidised glutathione (GSSG) and normalised GSH/GSSG ratio. : Both DHA-rich fish oil and TH have protective effects against brain oxidative stress but consuming these substances together does not seem to provide an additional benefit compared to consuming them separately.
长期暴露于慢性应激会引发氧化损伤，从而改变大脑中抗氧化和促氧化活性之间的动态平衡。特琅蜂蜜（TH）是一种马来西亚野生多花蜂蜜，已被证明含有大量抗氧化剂。富含DHA的鱼油是鱼类中发现的一种ω-3脂肪酸形式，也具有一定的抗氧化活性。本研究旨在评估富含DHA的鱼油、特琅蜂蜜及其组合对慢性应激大鼠模型氧化应激若干参数的抗应激活性。
将50只雄性Sprague Dawley大鼠分为（i）对照组，（ii）应激暴露组，（iii）应激暴露并给予特琅蜂蜜治疗组（每日两次，1 g/kg体重），（iv）应激暴露并给予富含DHA的鱼油治疗组（每日两次，450 mg/kg体重），以及（v）应激暴露并给予特琅蜂蜜和富含DHA的鱼油组合治疗组。慢性应激方案包括束缚应激和游泳应激试验相结合，持续28天。
富含DHA的鱼油和特琅蜂蜜显著（p < 0.05）抑制了应激诱导的血清皮质酮升高和脂质过氧化，并使总抗氧化能力显著增加。对于谷胱甘肽状态，只有特琅蜂蜜显著降低了应激诱导的氧化型谷胱甘肽（GSSG）升高，并使GSH/GSSG比值恢复正常。富含DHA的鱼油和特琅蜂蜜均对大脑氧化应激具有保护作用，但与单独食用相比，同时食用这些物质似乎并未提供额外益处。
