Department of Rehabilitation Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan, 610041, People's Republic of China.
Department of Rehabilitation Medicine, Yongchuan Hospital of Chongqing Medical University, Chongqing, 402160, People's Republic of China.
Metab Brain Dis. 2017 Dec;32(6):1805-1818. doi: 10.1007/s11011-017-0068-3. Epub 2017 Jul 13.
Ischemic postconditioning (IPostC) has been reported to have neuroprotection against ischemic diseases, and one cycle of IPostC induces neurogenesis when treated nearby. To expanding these effects, we explored the effects of repetitively remote IPostC (NRIPostC) on neurogenesis in the subgranular zone (SGZ) and subentricular zone (SVZ) during stroke recovery. Animals underwent transient cerebral ischemia were treated with vehicle or NRIPostC immediately after reperfusion. Neurological severity scores, infarct size, neurogenesis, and protein expression levels of nestin and GFAP were quantified at 3d, 7d, 14d, 21d and 28d post-ischemia. Results showed that NRIPostC significantly reduced acute infarction and improved neurological outcomes during the recovery phase. Meanwhile, NRIPostC significantly increased the number of BrdU/nestin cells in SGZ on day 14 and in the SVZ on days 3, 7 and 14 respectively, and the number of DCX cells from days 3 to 14. There were significant increments in the number of BrdU/NeuN and BrdU/GFAP cells in the SGZ and SVZ during the stroke recovery. The changing tendency of the protein expression of nestin and GFAP in DG was consistent with the result mentioned above. In conclusion, NRIPostC reduced acute infarction and improved functional outcomes up to 28d, and it induced neurogenesis both in the SGZ and SVZ.
缺血后处理（IPostC）已被报道具有对抗缺血性疾病的神经保护作用，并且在附近治疗时，一个周期的 IPostC 可诱导神经发生。为了扩大这些作用，我们探索了重复远程 IPostC（NRIPostC）对中风恢复期间颗粒下区（SGZ）和侧脑室下区（SVZ）神经发生的影响。动物在短暂性脑缺血后接受治疗，在再灌注后立即给予载体或 NRIPostC。在缺血后 3d、7d、14d、21d 和 28d 时，定量测定神经严重程度评分、梗死面积、神经发生以及巢蛋白和 GFAP 的蛋白表达水平。结果表明，NRIPostC 可显著减少急性梗死并改善恢复阶段的神经功能结果。同时，NRIPostC 可显著增加 SGZ 中 BrdU/nestin 细胞的数量（第 14 天）和 SVZ 中 BrdU/nestin 细胞的数量（第 3 天、第 7 天和第 14 天），以及 DCX 细胞的数量从第 3 天到第 14 天。在中风恢复期间，SGZ 和 SVZ 中 BrdU/NeuN 和 BrdU/GFAP 细胞的数量均显著增加。DG 中巢蛋白和 GFAP 蛋白表达的变化趋势与上述结果一致。总之，NRIPostC 可减少急性梗死并改善 28d 的功能结果，并可诱导 SGZ 和 SVZ 中的神经发生。
