Petruczynik Anna, Misiurek Justyna, Tuzimski Tomasz, Waksmundzka-Hajnos Monika
Medical University of Lublin, Department of Inorganic Chemistry, Chodźki 4a, 20-093 Lublin, Poland.
Medical University of Lublin, Department of Physical Chemistry, Chodźki 4a, 20-093 Lublin, Poland.
J AOAC Int. 2017 Nov 1;100(6):1652-1659. doi: 10.5740/jaoacint.17-0211. Epub 2017 Jul 13.
An HPLC procedure on a polar reversed-phase column with mobile phases containing ionic liquid (IL) was developed for the analysis of selected alkaloids from different chemical groups. We aimed to obtain optimal conditions for the separation of alkaloids because widely used silica-based stationary phases exhibit a silanol effect, rendering analysis of basic analytes extremely difficult. Retention, separation selectivity, peak symmetry, and system efficiency were examined in various eluent systems containing different concentrations of IL and acetonitrile. The obtained results revealed substantial influence from the concentrations of IL, the organic modifier, and temperature on the retention behavior of the investigated alkaloids. The most selective and efficient chromatographic systems were applied for the analysis of several alkaloids in a plant extract.
开发了一种在极性反相柱上使用含离子液体（IL）流动相的高效液相色谱法，用于分析来自不同化学组的选定生物碱。我们旨在获得生物碱分离的最佳条件，因为广泛使用的基于硅胶的固定相会表现出硅醇效应，使得碱性分析物的分析极其困难。在含有不同浓度IL和乙腈的各种洗脱液系统中考察了保留、分离选择性、峰对称性和系统效率。所得结果表明，IL浓度、有机改性剂和温度对所研究生物碱的保留行为有显著影响。最具选择性和高效的色谱系统用于分析植物提取物中的几种生物碱。
