Selby-Pham Sophie N B, Cottrell Jeremy J, Dunshea Frank R, Ng Ken, Bennett Louise E, Howell Kate S
Faculty of Veterinary and Agricultural, The University of Melbourne, Parkville, VIC 3010, Australia.
CSIRO Agriculture and Food, 671 Sneydes Road, Werribee, VIC 3010, Australia.
Nutrients. 2017 Jul 14;9(7):758. doi: 10.3390/nu9070758.
Phytochemical-rich diets are protective against chronic diseases and mediate their protective effect by regulation of oxidative stress (OS). However, it is proposed that under some circumstances, phytochemicals can promote production of reactive oxygen species (ROS) in vitro, which might drive OS-mediated signalling. Here, we investigated the effects of administering single doses of extracts of red cabbage and grape skin to pigs. Blood samples taken at baseline and 30 min intervals for 4 hours following intake were analyzed by measures of antioxidant status in plasma, including Trolox equivalent antioxidant capacity (TEAC) and glutathione peroxidase (GPx) activity. In addition, dose-dependent production of hydrogen peroxide (H₂O₂) by the same extracts was measured in untreated commercial pig plasma in vitro. Plasma from treated pigs showed extract dose-dependent increases in non-enzymatic (plasma TEAC) and enzymatic (GPx) antioxidant capacities. Similarly, extract dose-dependent increases in H₂O₂ were observed in commercial pig plasma in vitro. The antioxidant responses to extracts by treated pigs were highly correlated with their respective yields of H₂O₂ production in vitro. These results support that dietary phytochemicals regulate OS via direct and indirect antioxidant mechanisms. The latter may be attributed to the ability to produce H₂O₂ and to thereby stimulate cellular antioxidant defence systems.
富含植物化学物质的饮食对慢性疾病具有保护作用，并通过调节氧化应激（OS）来介导其保护作用。然而，有人提出，在某些情况下，植物化学物质在体外可促进活性氧（ROS）的产生，这可能会驱动由OS介导的信号传导。在此，我们研究了给猪单次投喂红甘蓝和葡萄皮提取物的效果。在摄入后4小时内，每隔30分钟采集一次血样，并通过测量血浆中的抗氧化状态进行分析，包括特洛克斯等效抗氧化能力（TEAC）和谷胱甘肽过氧化物酶（GPx）活性。此外，在未处理的商业猪血浆中体外测量相同提取物产生过氧化氢（H₂O₂）的剂量依赖性。处理过的猪的血浆显示，非酶（血浆TEAC）和酶（GPx）抗氧化能力呈提取物剂量依赖性增加。同样，在商业猪血浆体外观察到提取物剂量依赖性增加H₂O₂。处理过的猪对提取物的抗氧化反应与其在体外各自产生H₂O₂的产量高度相关。这些结果支持饮食中的植物化学物质通过直接和间接的抗氧化机制调节OS。后者可能归因于产生H₂O₂的能力，从而刺激细胞抗氧化防御系统。
