Nunes Emily M, López Rossana V M, Sudenga Staci L, Gheit Tarik, Tommasino Massimo, Baggio Maria L, Ferreira Silvaneide, Galan Lenice, Silva Roberto C, Lazcano-Ponce Eduardo, Giuliano Anna R, Villa Luisa L, Sichero Laura
Molecular Biology Laboratory, Center for Translational Research in Oncology, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
Department of Epidemiology, Center for Translational Research in Oncology, Instituto do Câncer do Estado de São Paulo, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
Virology. 2017 Oct;510:55-59. doi: 10.1016/j.virol.2017.07.006. Epub 2017 Jul 11.
We evaluated the concordance between β-HPVs detected in external genital skin, anal canal, and oral cavity specimens collected simultaneously from 717 men that were participating in the multinational HIM Study. Viral genotyping was performed using the Luminex technology. Species- and type-specific concordance was measured using kappa statistics for agreement. Overall, concordance of β-HPVs across sites was low and mainly observed among paired genital/anal canal samples. When grouped by species, solely β-4 HPVs showed moderate concordance in genital/anal pairs (κ = 0.457), which could be attributed to the substantial concordance of HPV-92 in men from Brazil and Mexico (κ > 0.610). β-HPV type concordance was higher in Mexico, where HPV-19 was consistently concordant in all anatomic site combinations. Our analysis indicates that type-specific concordance across sites is limited to few viral types; however, these infections seem to occur more often than would be expected by chance, suggesting that although rare, there is agreement among sites.
我们评估了在参与多国HIM研究的717名男性中同时采集的外生殖器皮肤、肛管和口腔标本中检测到的β-人乳头瘤病毒（β-HPVs）之间的一致性。使用Luminex技术进行病毒基因分型。使用kappa一致性统计量来测量种属特异性和型特异性的一致性。总体而言，β-HPVs在不同部位之间的一致性较低，主要在配对的生殖器/肛管样本中观察到。按种属分组时，仅β-4 HPVs在生殖器/肛管配对样本中显示出中度一致性（κ = 0.457），这可能归因于来自巴西和墨西哥的男性中HPV-92的高度一致性（κ > 0.610）。在墨西哥，β-HPV型的一致性更高，其中HPV-19在所有解剖部位组合中均始终保持一致。我们的分析表明，不同部位之间的型特异性一致性仅限于少数病毒类型；然而，这些感染似乎比偶然预期的更频繁发生，这表明尽管罕见，但不同部位之间存在一致性。
