Nascimento de Souza Raquel, Silva Fernanda Kohn, Alves de Medeiros Magda
Department of Physiological Sciences, Federal Rural University of Rio de Janeiro (UFRRJ), Seropedica, RJ, Brazil.
Department of Physiological Sciences, Federal Rural University of Rio de Janeiro (UFRRJ), Seropedica, RJ, Brazil; Multicenter Post-graduation Program in Physiological Sciences, Brazilian Society of Physiology, São Paulo, SP, Brazil; Post-graduation Program in Physiological Sciences of Federal Rural University of Rio de Janeiro, Seropedica, RJ, Brazil.
J Acupunct Meridian Stud. 2017 Jun;10(3):204-210. doi: 10.1016/j.jams.2017.04.003. Epub 2017 Apr 21.
Spinal cord injuries (SCIs) initiate a series of molecular and cellular events in which inflammatory responses can lead to major neurological dysfunctions. The present study aims to investigate whether bee venom (BV) acupuncture applied at acupoints ST36 (Zusanli) and GV3 (Yaoyangquan) could minimize locomotor deficits and the magnitude of neural tissue losses, and change the balance between pro- and anti-inflammatory cytokines after an SCI by compression. Wistar rats were subjected to an SCI model by compression in which a 2-French Fogarty embolectomy catheter was inflated in the extradural space. The effects of BV acupuncture, in which 20 μL of BV diluted in saline (0.08 mg/kg) was injected at acupoints GV3 and ST36 [BV(ST36+GV3)-SCI] was compared with BV injected at nonacupoints [BV(NP)-SCI] and with no treatment [group subjected only to SCI (CTL-SCI)]. The BV(ST36+GV3)-SCI group showed a significant improvement in the locomotor performance and a decrease of lesion size compared with the controls. BV acupuncture at the ST36 + GV3 increased the expression of interleukin-10 (anti-inflammatory) at 6 hours and reduced the expression of interleukin-6 (proinflammatory) at 24 hours after SCI compared with the controls. Our results suggest that BV acupuncture can reduce neuroinflammation and induce recovery in the SCI compression model.
脊髓损伤（SCIs）会引发一系列分子和细胞事件，其中炎症反应可导致严重的神经功能障碍。本研究旨在探讨针刺足三里（ST36）和腰阳关（GV3）穴位的蜂毒（BV）是否能减轻运动功能障碍和神经组织损失的程度，并改变脊髓压迫损伤后促炎细胞因子和抗炎细胞因子之间的平衡。将Wistar大鼠制成脊髓压迫损伤模型，在硬膜外腔插入一根2F的Fogarty取栓导管并充气。将在GV3和ST36穴位注射20μL生理盐水稀释的BV（0.08mg/kg）的蜂毒针刺组[BV(ST36+GV3)-SCI]与在非穴位注射BV的组[BV(NP)-SCI]以及未治疗组[仅接受脊髓损伤的组（CTL-SCI）]进行比较。与对照组相比，BV(ST36+GV3)-SCI组的运动性能有显著改善，损伤大小减小。与对照组相比，在脊髓损伤后6小时，针刺ST36 + GV3穴位的蜂毒可增加白细胞介素-10（抗炎）的表达，在24小时可降低白细胞介素-6（促炎）的表达。我们的结果表明，蜂毒针刺可减轻脊髓压迫损伤模型中的神经炎症并促进恢复。
