Randriamboavonjy Joseph Iharinjaka, Rio Marc, Pacaud Pierre, Loirand Gervaise, Tesse Angela
INSERM, CNRS, Université de Nantes, l'institut du thorax, 44000 Nantes, France.
CHU de Nantes, 44000 Nantes, France.
Oxid Med Cell Longev. 2017;2017:4129459. doi: 10.1155/2017/4129459. Epub 2017 Jun 20.
(MOI) is a tree currently used in traditional medicine in tropical Africa, America, and Asia for therapeutic applications in several disorders including arterial hypertension. We previously described a cardiac protective role of a treatment with MOI seeds in spontaneously hypertensive rats (SHR). Here, we investigated the effects of this treatment on oxidative and nitrosative vascular stresses in SHR, with normotensive Wistar Kyoto rats used as controls. Oxidative and nitrosative stresses detected in SHR aortas using the fluorescent dye dihydroethidine and protein nitrotyrosine staining were reduced in MOI-treated SHR aortas. This was associated with a decrease of free 8-isoprostane circulating level, vascular p22 and p47 expressions, and SOD2 upregulation. Moreover, circulating nitrites and C-reactive protein, increased in SHR, were both reduced in SHR receiving MOI. This was associated to decrease iNOS and NF-B protein expressions after MOI treatment. In functional studies, the endothelium-dependent carbachol-induced relaxation was improved in MOI-treated SHR resistance arteries. Oral administration of MOI seeds demonstrates vascular antioxidant, anti-inflammatory, and endothelial protective effects in SHR. Our data support the use of MOI seeds in diet against cardiovascular disorders associated with oxidative stress and inflammation such as hypertension, scientifically validating the use of these seeds in Malagasy traditional medicine.
（MOI）是一种树，目前在热带非洲、美洲和亚洲的传统医学中用于治疗包括动脉高血压在内的多种疾病。我们之前描述了用MOI种子治疗对自发性高血压大鼠（SHR）的心脏保护作用。在此，我们研究了这种治疗对SHR氧化和亚硝化血管应激的影响，并以血压正常的Wistar Kyoto大鼠作为对照。使用荧光染料二氢乙锭和蛋白质硝基酪氨酸染色在SHR主动脉中检测到的氧化和亚硝化应激在接受MOI治疗的SHR主动脉中降低。这与游离8-异前列腺素循环水平降低、血管p22和p47表达降低以及SOD2上调有关。此外，SHR中升高的循环亚硝酸盐和C反应蛋白在接受MOI的SHR中均降低。这与MOI治疗后iNOS和NF-κB蛋白表达降低有关。在功能研究中，接受MOI治疗的SHR阻力动脉中内皮依赖性卡巴胆碱诱导的舒张功能得到改善。口服MOI种子对SHR具有血管抗氧化、抗炎和内皮保护作用。我们的数据支持在饮食中使用MOI种子来对抗与氧化应激和炎症相关的心血管疾病，如高血压，从科学上验证了这些种子在马达加斯加传统医学中的应用。
