Gupta Vasundhera, Singh Pradip Kumar, Korpole Suresh, Tanuku Naga Radha Srinivas, Pinnaka Anil Kumar
MTCC-Microbial Type Culture Collection & Gene Bank, CSIR-Institute of Microbial Technology, Chandigarh-160036, India.
CSIR-National Institute of Oceanography, Regional Centre, 176, Lawsons Bay Colony, Visakhapatnam-530017, India.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2219-2224. doi: 10.1099/ijsem.0.001928. Epub 2017 Jul 17.
A facultatively anaerobic, endospore forming, alkali-tolerant, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK61T, was isolated from a sediment sample collected from Coringa mangrove forest, India. Colonies were circular, 1.5 mm in diameter, shiny, smooth, yellowish and convex with entire margins after 48 h growth at 30 °C. Growth occurred at 15-42 °C, with 0-3 % (w/v) NaCl and at pH 6-9. AK61T was positive for amylase activity and negative for oxidase, catalase, aesculinase, caseinase, cellulase, DNase, gelatinase, lipase and urease activities. The fatty acids were dominated by branched types with iso- and anteiso- saturated fatty acids with a high abundance of iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0; the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid; and MK-7 was the major menaquinone. DNA-DNA hybridization between AK61T and Bacillus indicus MTCC 4374T and between AK61T and Bacillus indicus KCTC 3880 showed relatedness of 37.99 and 33.32 % respectively. The DNA G+C content of AK61T was 44 mol%. The results of a blast sequence similarity search based on 16S rRNA gene sequences indicated that Bacillus cibi and Bacillus indicus were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 97.69 and 97.55 % respectively. The results of phylogenetic analysis indicated that AK61T was clustered with Bacillus idriensis and Bacillus indicus. On the basis of its phenotypic characteristics and phylogenetic inference, AK61T represents a novel species of the genus Bacillus, for which the name Bacillus mangrovi sp. nov. is proposed. The type strain is AK61T (=JCM 31087T=MTCC 12015T=KCTC 33872T).
从印度科林加红树林采集的沉积物样本中分离出一株兼性厌氧、形成芽孢、耐碱、革兰氏染色阳性、具运动性的杆状细菌，命名为AK61T菌株。在30°C下培养48小时后，菌落呈圆形，直径1.5毫米，有光泽、光滑、淡黄色且边缘整齐凸起。该菌在15-42°C、0-3%（w/v）NaCl和pH值6-9的条件下生长。AK61T菌株淀粉酶活性呈阳性，氧化酶、过氧化氢酶、七叶苷酶、酪蛋白酶、纤维素酶、DNA酶、明胶酶、脂肪酶和脲酶活性呈阴性。脂肪酸以支链类型为主，包括异饱和脂肪酸和anteiso饱和脂肪酸，其中异-C14 : 0、异-C15 : 0、anteiso-C15 : 0和异-C16 : 0含量较高；细胞壁肽聚糖含有内消旋二氨基庚二酸作为诊断性二氨基酸；MK-7是主要的甲基萘醌。AK61T与印度芽孢杆菌MTCC 4,374T以及AK61T与印度芽孢杆菌KCTC 3,880之间的DNA-DNA杂交相似度分别为37.99%和33.32%。AK61T菌株的DNA G+C含量为44摩尔%。基于16S rRNA基因序列的blast序列相似性搜索结果表明，食源芽孢杆菌和印度芽孢杆菌是最近的系统发育邻种，序列相似性分别为97.69%和97.55%。系统发育分析结果表明，AK61T与伊德里芽孢杆菌和印度芽孢杆菌聚类在一起。基于其表型特征和系统发育推断，AK61T代表芽孢杆菌属的一个新物种，为此提出名为红树林芽孢杆菌（Bacillus mangrovi sp. nov.）的新名称。模式菌株为AK61T（=JCM 31,087T=MTCC 12,015T=KCTC 33,872T）。
