Singh Harjodh, Kaur Manpreet, Kaur Lakhwinder, Sharma Shivani, Mishra Sunita, Tanuku Naga Radha Srinivas, Pinnaka Anil Kumar
Academy of Scientific and Innovative Research, (AcSIR), CSIR Campus, Chennai, India.
MTCC-Microbial Type Culture Collection and Gene Bank, CSIR-Institute of Microbial Technology, Chandigarh 160036, India.
Int J Syst Evol Microbiol. 2018 Mar;68(3):801-809. doi: 10.1099/ijsem.0.002588.
A strictly aerobic, alkaliphilic, Gram-stain-positive, motile, rod-shaped bacterium, designated strain AK74, was isolated from a water sample collected from Sambhar salt lake, Rajasthan, India. Colonies were circular, 1.2 mm in diameter, shiny, smooth, whitish and convex with an entire margin after 48 h growth at 37 °C with pH 9.0. Growth occurred at 25-42 °C, 0-4 % (w/v) NaCl and at a pH of 7-12. Strain AK74 was positive for aesculinase, caseinase, lipase activities and negative for oxidase, catalase, amylase, cellulase, DNase, gelatinase and urease activities. The fatty acids were dominated by branched iso-, anteiso- and saturated fatty acids with a high abundance of iso-C, anteiso-C, C and C and the cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The DNA G+C content of strain AK74 was 51.6 mol%. A blast sequence similarity search based on 16S rRNA gene sequences indicated that , and were the nearest phylogenetic neighbours, with a pair-wise sequence similarities of 96.6, 96.6 and 96.5%, respectively. Phylogenetic analysis showed that strain AK74 clustered with and together clustered with and . Based on its phenotypic characteristics and on phylogenetic inference, strain AK74 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AK74 (=MTCC 12638=KCTC 33946=JCM 32185).
从印度拉贾斯坦邦桑巴尔盐湖采集的水样中分离出一株严格需氧、嗜碱、革兰氏染色阳性、运动、杆状的细菌，命名为AK74菌株。在37°C、pH 9.0条件下培养48小时后，菌落呈圆形，直径1.2毫米，有光泽、光滑、带白色且凸起，边缘整齐。该菌株在25-42°C、0-4%（w/v）NaCl以及pH 7-12的条件下生长。AK74菌株对七叶灵酶、酪蛋白酶、脂肪酶呈阳性反应，对氧化酶、过氧化氢酶、淀粉酶、纤维素酶、DNA酶、明胶酶和脲酶呈阴性反应。脂肪酸以支链异、anteiso和饱和脂肪酸为主，其中异-C、anteiso-C、C和C含量较高，细胞壁肽聚糖含有-diaminopimelic acid作为诊断性二氨基酸。AK74菌株的DNA G+C含量为51.6摩尔%。基于16S rRNA基因序列的blast序列相似性搜索表明， 、 和 是最近的系统发育邻居，两两序列相似性分别为96.6%、96.6%和96.5%。系统发育分析表明，AK74菌株与 聚类， 与 和 一起聚类。基于其表型特征和系统发育推断，AK74菌株代表了 属的一个新物种，为此提出新物种名称 sp. nov.。模式菌株为AK74（=MTCC 12638=KCTC 33946=JCM 32185）。
