School of Agriculture and Food Engineering, Shandong University of Technology, Zibo, People's Republic of China.
Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No. 12 Zhangzhou Road, Zibo, 255049, Shandong, People's Republic of China.
Bioprocess Biosyst Eng. 2017 Sep;40(9):1419-1425. doi: 10.1007/s00449-017-1799-6. Epub 2017 Jul 17.
In this study an impedance aptasensor was designed for sensitive, selective, and fast detection of tetracycline (TET) based on an interdigital array microelectrode (IDAM). The IDAM was integrated with impedance detection to miniaturize the conventional electrodes, enhance the sensitivity, shorten the detection time, and minimize interfering effects of non-target analytes in the solution. Due to their excellent conductivity, good biocompatibility, the multi-walled carbon nanotubes (MWCNTs) were used to modify the IDAM to immobilize TET aptamer effectively. The proposed aptasensor produced a sensitive impedance change which was characterized by the electrochemical impedance spectroscopy (EIS). With the addition of TET, the formation of TET-aptamer complex on the surface of MWCNTs modified electrode resulted in an increase of electron transfer resistance (R ). The change of R depends on the concentration of TET, which is applied for the quantification of TET. A wide linear range was obtained from 10 to 10 M. The linear regression equation was y(ΔR) = 21.310 × x(LogC) (M) + 217.25. It was successfully applied to detect TET in real milk samples.
在这项研究中，设计了一种基于叉指式微电极（IDAM）的阻抗适体传感器，用于灵敏、选择性和快速检测四环素（TET）。IDAM 与阻抗检测相结合，可使传统电极微型化，提高灵敏度，缩短检测时间，并最小化溶液中非目标分析物的干扰影响。由于多壁碳纳米管（MWCNTs）具有优良的导电性、良好的生物相容性，因此被用于修饰 IDAM 以有效固定 TET 适体。所提出的适体传感器产生了灵敏的阻抗变化，其特征在于电化学阻抗谱（EIS）。随着 TET 的加入，TET-适体复合物在 MWCNTs 修饰电极表面的形成导致电子转移电阻（R ）增加。R 的变化取决于 TET 的浓度，可用于 TET 的定量。从 10 到 10 M 获得了宽线性范围。线性回归方程为 y(ΔR) = 21.310×x(LogC) (M) + 217.25。它成功地应用于检测实际牛奶样品中的 TET。
