• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于皮克级四环素检测的选择性抗体免费传感膜。

Selective Antibody-Free Sensing Membranes for Picogram Tetracycline Detection.

机构信息

Institut de Sciences Analytiques (ISA)-UMR 5280, Université Claude Bernard Lyon 1, 5 Rue de la Doua, 69100 Lyon, France.

Inorganic Materials Laboratory, Institut de Ciencia de Materials de Barcelona (ICMAB-CSIC), Campus de la UAB, Bellaterra, 08193 Barcelona, Spain.

出版信息

Biosensors (Basel). 2022 Dec 31;13(1):71. doi: 10.3390/bios13010071.

DOI:10.3390/bios13010071
PMID:36671906
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9855611/
Abstract

As an antibody-free sensing membrane for the detection of the antibiotic tetracycline (TC), a liquid PVC membrane doped with the ion-pair tetracycline/θ-shaped anion [3,3'-Co(1,2-CBH)] ([-COSAN]) was formulated and deposited on a SWCNT modified gold microelectrode. The chosen transduction technique was electrochemical impedance spectroscopy (EIS). The PVC membrane was composed of: the tetracycline/[-COSAN] ion-pair, a plasticizer. A detection limit of 0.3 pg/L was obtained with this membrane, using bis(2-ethylhexyl) sebacate as a plasticizer. The sensitivity of detection of tetracycline was five times higher than that of oxytetracycline and of terramycin, and 22 times higher than that of demeclocycline. A shelf-life of the prepared sensor was more than six months and was used for detection in spiked honey samples. These results open the way to having continuous monitoring sensors with a high detection capacity, are easy to clean, avoid the use of antibodies, and produce a direct measurement.

摘要

作为一种用于检测抗生素四环素（TC）的无抗体传感膜，我们制备了一种掺杂离子对四环素/θ 型阴离子[3,3'-Co(1,2-CBH)]（[-COSAN]）的液态 PVC 膜，并将其沉积在经过单壁碳纳米管修饰的金微电极上。选择的转换技术是电化学阻抗谱（EIS）。该 PVC 膜由：四环素/[-COSAN]离子对、增塑剂组成。使用癸二酸二异辛酯作为增塑剂时，该膜的检测限达到了 0.3 pg/L。检测四环素的灵敏度比土霉素和泰乐菌素高五倍，比去甲金霉素高 22 倍。制备的传感器的保质期超过六个月，并用于检测加标蜂蜜样品。这些结果为具有高检测能力、易于清洁、避免使用抗体和进行直接测量的连续监测传感器开辟了道路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/626a79f7201e/biosensors-13-00071-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/b094f6bdf1eb/biosensors-13-00071-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/a17a2ef04c70/biosensors-13-00071-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/46802e54528a/biosensors-13-00071-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/134917a36d29/biosensors-13-00071-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/dca599222a05/biosensors-13-00071-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/1bedb935a933/biosensors-13-00071-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/519fba24a560/biosensors-13-00071-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/626a79f7201e/biosensors-13-00071-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/b094f6bdf1eb/biosensors-13-00071-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/a17a2ef04c70/biosensors-13-00071-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/46802e54528a/biosensors-13-00071-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/134917a36d29/biosensors-13-00071-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/dca599222a05/biosensors-13-00071-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/1bedb935a933/biosensors-13-00071-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/519fba24a560/biosensors-13-00071-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78d6/9855611/626a79f7201e/biosensors-13-00071-g008.jpg

相似文献

1
Selective Antibody-Free Sensing Membranes for Picogram Tetracycline Detection.用于皮克级四环素检测的选择性抗体免费传感膜。
Biosensors (Basel). 2022 Dec 31;13(1):71. doi: 10.3390/bios13010071.
2
Polymeric plasticizer extends the lifetime of PVC-membrane ion-selective electrodes.聚合物增塑剂延长了 PVC 膜离子选择性电极的寿命。
Analyst. 2014 Feb 21;139(4):757-63. doi: 10.1039/c3an01963b. Epub 2013 Dec 18.
3
An interdigital array microelectrode aptasensor based on multi-walled carbon nanotubes for detection of tetracycline.基于多壁碳纳米管的叉指式微电极适体传感器用于检测四环素。
Bioprocess Biosyst Eng. 2017 Sep;40(9):1419-1425. doi: 10.1007/s00449-017-1799-6. Epub 2017 Jul 17.
4
The Influence of Plasticizers on the Response Characteristics of the Surfactant Sensor for Cationic Surfactant Determination in Disinfectants and Antiseptics.增塑剂对消毒剂和防腐剂中阳离子表面活性剂测定用表面活性剂传感器响应特性的影响。
Sensors (Basel). 2021 May 19;21(10):3535. doi: 10.3390/s21103535.
5
Finding a perfect match of ion-exchanger and plasticizer for ion-selective sensors.为离子选择性传感器寻找离子交换剂和增塑剂的完美匹配物。
Talanta. 2024 Mar 1;269:125408. doi: 10.1016/j.talanta.2023.125408. Epub 2023 Nov 20.
6
Hybrid Plasticizers Enhance Specificity and Sensitivity of an Electrochemical-Based Sensor for Cadmium Detection.混合增塑剂提高基于电化学传感器检测镉的特异性和灵敏度。
Int J Mol Sci. 2022 Jun 8;23(12):6402. doi: 10.3390/ijms23126402.
7
Eco-friendly electrochemical assay of oxytetracycline and flunixin in their veterinary injections and spiked milk samples.兽用注射剂及加标牛奶样品中土霉素和氟尼辛的环保型电化学检测
BMC Chem. 2024 Sep 19;18(1):179. doi: 10.1186/s13065-024-01282-4.
8
A newly synthesized macrocyclic dithioxamide receptor for phosphate sensing.一种新合成的用于磷酸盐传感的大环二硫代乙酰胺受体。
Talanta. 2006 Jun 15;69(4):1007-12. doi: 10.1016/j.talanta.2005.12.023. Epub 2006 Jan 10.
9
Comparison of two fabricated aptasensors based on modified carbon paste/oleic acid and magnetic bar carbon paste/Fe3O4@oleic acid nanoparticle electrodes for tetracycline detection.基于修饰碳糊/油酸和磁性棒碳糊/Fe3O4@油酸纳米粒子电极的两种制备适体传感器用于四环素检测的比较。
Biosens Bioelectron. 2016 Nov 15;85:553-562. doi: 10.1016/j.bios.2016.05.052. Epub 2016 May 17.
10
Dual-response fluorescent probe based on nitrogen-doped carbon dots and europium ions hybrid for ratiometric and on-site visual determination of oxytetracycline and tetracycline.基于氮掺杂碳点和铕离子杂化的双响应荧光探针用于比率和现场可视化测定土霉素和四环素。
Sci Total Environ. 2023 Feb 20;860:160533. doi: 10.1016/j.scitotenv.2022.160533. Epub 2022 Nov 28.

引用本文的文献

1
(Bio)Electroanalysis of Tetracyclines: Recent Developments.四环素的（生物）电分析：最新进展
Biosensors (Basel). 2025 Feb 11;15(2):101. doi: 10.3390/bios15020101.
2
Towards the Application of Purely Inorganic Icosahedral Boron Clusters in Emerging Nanomedicine.迈向纯无机二十面体硼簇在新兴纳米医学中应用的研究。
Molecules. 2023 May 30;28(11):4449. doi: 10.3390/molecules28114449.

本文引用的文献

1
Immuno field-effect transistor (ImmunoFET) for detection of salivary cortisol using potentiometric and impedance spectroscopy for monitoring heart failure.免疫场效应晶体管（ImmunoFET）用于检测唾液皮质醇，采用电势和阻抗谱法监测心力衰竭。
Talanta. 2023 May 15;257:123802. doi: 10.1016/j.talanta.2022.123802. Epub 2022 Sep 15.
2
A Novel Cortisol Immunosensor Based on a Hafnium Oxide/Silicon Structure for Heart Failure Diagnosis.一种基于氧化铪/硅结构的用于心力衰竭诊断的新型皮质醇免疫传感器。
Micromachines (Basel). 2022 Dec 16;13(12):2235. doi: 10.3390/mi13122235.
3
A novel electrochemical strategy for NT-proBNP detection using IMFET for monitoring heart failure by saliva analysis.
一种使用离子敏场效应晶体管（IMFET）通过唾液分析监测心力衰竭来检测N末端B型利钠肽原（NT-proBNP）的新型电化学策略。
Talanta. 2023 Jan 1;251:123759. doi: 10.1016/j.talanta.2022.123759. Epub 2022 Aug 5.
4
A silicon nitride ISFET based immunosensor for tumor necrosis factor-alpha detection in saliva. A promising tool for heart failure monitoring.一种用于检测唾液中肿瘤坏死因子-α的基于氮化硅离子敏感场效应晶体管的免疫传感器。一种用于心力衰竭监测的有前景的工具。
Anal Chim Acta. 2021 May 29;1161:338468. doi: 10.1016/j.aca.2021.338468. Epub 2021 Apr 2.
5
Development of an electrochemical biosensor for impedimetric detection of tetracycline in milk.用于牛奶中四环素阻抗检测的电化学生物传感器的研制。
J Food Sci Technol. 2020 Dec;57(12):4697-4706. doi: 10.1007/s13197-020-04506-2. Epub 2020 May 9.
6
Atomistic Simulations of COSAN: Amphiphiles without a Head-and-Tail Design Display "Head and Tail" Surfactant Behavior.无头-尾设计两亲分子 COSAN 的原子级模拟：表现出头尾型表面活性剂行为。
Angew Chem Int Ed Engl. 2020 Feb 17;59(8):3088-3092. doi: 10.1002/anie.201913257. Epub 2020 Jan 3.
7
Development and application of a novel electrochemical immunosensor for tetracycline screening in honey using a fully integrated electrochemical Bio-MEMS.基于完全集成电化学 Bio-MEMS 的新型电化学免疫传感器在蜂蜜中四环素筛选的开发与应用。
Biosens Bioelectron. 2019 Apr 1;130:330-337. doi: 10.1016/j.bios.2018.09.052. Epub 2018 Sep 13.
8
Label-free photoelectrochemical aptasensor for tetracycline detection based on cerium doped CdS sensitized BiYWO.基于掺铈的 CdS 敏化 BiYWO 的无标记光电流型适体传感器用于四环素的检测
Biosens Bioelectron. 2018 May 30;106:7-13. doi: 10.1016/j.bios.2018.01.051. Epub 2018 Jan 31.
9
Determination of five tetracyclines and their epimers by LC-MS/MS based on a liquid-liquid extraction with low temperature partitioning.基于低温分配液液萃取的液相色谱-串联质谱法测定五种四环素及其差向异构体
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2018 Apr;35(4):686-694. doi: 10.1080/19440049.2018.1427894. Epub 2018 Feb 20.
10
An interdigital array microelectrode aptasensor based on multi-walled carbon nanotubes for detection of tetracycline.基于多壁碳纳米管的叉指式微电极适体传感器用于检测四环素。
Bioprocess Biosyst Eng. 2017 Sep;40(9):1419-1425. doi: 10.1007/s00449-017-1799-6. Epub 2017 Jul 17.