Hunan Province Key Laboratory of Food Science and Biotechnology, Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, College of Food Science and Technology, Hunan Agricultural University, Changsha, 410128, People's Republic of China.
Mikrochim Acta. 2019 Aug 22;186(9):637. doi: 10.1007/s00604-019-3718-9.
A method is reported for in-situ detection of the antibiotic tetracycline (TC). It is based on a combination of extraction of TC by magnetic molecularly imprinted polymers nanoparticles (MMIPs NPs) and detection by surface plasmon resonance (SPR). The TC-captured MMIPs NPs were flowed over the surface of the SPR chip that was modified with mercaptoethylamine. The SPR signal undergoes a strong increase through the use of MMIPs NPs. It increases linearly in the 5.0-100 pg·mL TC concentration range, and the detection limit is as low as 1.0 pg·mL (at S/N = 3). This method shows selectivity to TC compared with structurally analogues. In order to demonstrate the power of the method, it was applied to the analysis of milk spiked with TC. These results were validated by comparing them to those of an enzyme-linked immunoassay. The average recovery is in the range of 95.7-104.6%. Graphical abstract Schematic representation of a surface plasmon resonance assay for the sensitive determination of tetracycline in milk using the magnetic molecularly imprinted polymers nanoparticles that can extract tetracycline and amplify the SPR signal.
报道了一种用于原位检测抗生素四环素（TC）的方法。它基于磁分子印迹聚合物纳米粒子（MMIPs NPs）萃取 TC 和表面等离子体共振（SPR）检测的结合。TC 捕获的 MMIPs NPs 流经经过巯基乙胺修饰的 SPR 芯片表面。通过使用 MMIPs NPs，SPR 信号会发生强烈增加。它在 5.0-100 pg·mL TC 浓度范围内呈线性增加，检测限低至 1.0 pg·mL（信噪比为 3）。与结构类似物相比，该方法对 TC 表现出选择性。为了证明该方法的强大功能，将其应用于 TC 加标牛奶的分析。通过将这些结果与酶联免疫吸附测定的结果进行比较，对其进行了验证。平均回收率在 95.7-104.6%范围内。
