Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
J Genet Genomics. 2019 May 20;46(5):259-267. doi: 10.1016/j.jgg.2019.02.007. Epub 2019 Mar 22.
AGC protein kinases play important roles in plant growth and development. Several AGC kinases in Arabidopsis have been functionally characterized. However, the "AGC Other" subfamily, including IRE, IREH1, IRE3 and IRE4, has not been well understood. Here, we reported that ireh1 mutants displayed a root skewing phenotype, which can be enhanced by ire3 mutation. IREH1 and IRE3 were expressed in roots, consistent with their function in controlling root skewing. The fluorescence intensities of the microtubule marker KNpro:EGFP-MBD were decreased in ireh1, ire3 and ireh1 ire3 mutants compared to wild type. The microtubule arrangements in ireh1 and ireh1 ire3 mutants were also altered. IREH1 physically interacted with IRE3 in vitro and in planta. Thus, our findings demonstrate that IREH1 and IRE3 protein kinases play important roles in controlling root skewing, and maintaining microtubule network in Arabidopsis.
AGC 蛋白激酶在植物生长发育中发挥重要作用。拟南芥中的几种 AGC 激酶已被功能表征。然而，“AGC Other”亚家族，包括 IRE、IREH1、IRE3 和 IRE4，尚未得到很好的理解。在这里，我们报道了 ireh1 突变体表现出根倾斜表型，ire3 突变可增强该表型。IREH1 和 IRE3 在根中表达，与它们在控制根倾斜中的功能一致。与野生型相比，ireh1、ire3 和 ireh1 ire3 突变体中微管标记物 KNpro:EGFP-MBD 的荧光强度降低。ireh1 和 ireh1 ire3 突变体中的微管排列也发生了改变。IREH1 在体外和体内与 IRE3 发生物理相互作用。因此，我们的研究结果表明，IREH1 和 IRE3 蛋白激酶在控制根倾斜和维持拟南芥微管网络方面发挥重要作用。
