Blariza María J, Grosso Carla G, García Beatriz A
Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
Am J Trop Med Hyg. 2017 Aug;97(2):477-480. doi: 10.4269/ajtmh.16-1029. Epub 2017 Jul 19.
RNA interference caused by exogenous double-stranded RNA (dsRNA) is used to downregulate crucial genes to control insects. The reproductive success of all oviparous species depends on vitellogenin (Vg) biosynthesis and its accumulation in the developing oocytes. Adult females of were independently injected with two dsRNAs ( dsRNA or dsRNA) or nuclease-free water (control) 24 hours before feeding, and a group of adult females not injected was also analyzed (control). and messenger RNAs silencing was verified by reverse transcription quantitative polymerase chain reaction. The transcript levels of the and genes were significantly reduced after dsRNA treatment in fat body and ovary of in relation to those detected in individuals injected with nuclease-free water and not injected (controls). Moreover, the present study demonstrated that the silencing of the or genes inhibits oviposition in the Chagas disease vector . These findings may have important implications for the development of novel vector control strategies.
由外源双链RNA（dsRNA）引起的RNA干扰被用于下调关键基因以控制昆虫。所有卵生物种的繁殖成功都依赖于卵黄原蛋白（Vg）的生物合成及其在发育中的卵母细胞中的积累。在进食前24小时，将成年雌性 分别独立注射两种dsRNA（dsRNA或dsRNA）或无核酸酶水（对照），并且还分析了一组未注射的成年雌性（对照）。通过逆转录定量聚合酶链反应验证 和 信使RNA的沉默。与注射无核酸酶水和未注射的个体（对照）中检测到的水平相比，dsRNA处理后， 在脂肪体和卵巢中的 和 基因的转录水平显著降低。此外，本研究表明， 或 基因的沉默抑制了恰加斯病媒介 中的产卵。这些发现可能对新型病媒控制策略的开发具有重要意义。
