与恰加斯病传播媒介（半翅目：红蝽科）杀虫剂抗性相关基因的表达的日变化。
Daily Variations in the Expression of Genes Related to Insecticide Resistance in the Chagas Disease Vector (Hemiptera: Reduviidae).
机构信息
Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
出版信息
Am J Trop Med Hyg. 2019 Jun;100(6):1482-1485. doi: 10.4269/ajtmh.19-0155.
Pyrethroid resistance has been detected in , which in part has been attributed to increased oxidative metabolism of the insecticide by cytochrome P450s. Nicotinamide adenine dinucleotide phosphate (NADPH) cytochrome P450 reductase (CPR) catalyzes electron transfer from NADPH to all known cytochrome P450s. In this study, the expression of the gene at transcriptional level was determined in different tissues and two life stages. The expression patterns showed differences in the tissues and stages studied, suggesting differential metabolic requirements. On the other hand, to investigate the presence of rhythms in the expression of genes related with insecticide resistance, we explored the daily expression profile of the gene and a gene () in fat body from adults of under different dark/light regimes. The results suggest that gene expression is under endogenous clock regulation and show a rhythmic profile in the expression of the gene.
已在 中检测到拟除虫菊酯抗性，部分原因是细胞色素 P450s 对杀虫剂的氧化代谢增加。烟酰胺腺嘌呤二核苷酸磷酸 (NADPH) 细胞色素 P450 还原酶 (CPR) 催化从 NADPH 向所有已知细胞色素 P450 转移电子。在这项研究中，在不同组织和两个生命阶段测定了 基因在转录水平上的表达。表达模式显示出研究的组织和阶段的差异，表明代谢需求的差异。另一方面，为了研究与杀虫剂抗性相关基因表达的节律性，我们探索了在不同明暗条件下成虫脂肪体中 基因和一个 基因 ()的每日表达谱。结果表明， 基因表达受内源性时钟调节，并显示 基因表达的节律性。
Zotero 插件