Hooda Vinita, Gahlaut Anjum, Gothwal Ashish, Hooda Vikas
Centre for Biotechnology, Maharshi Dayanand University, Rohtak, Haryana, 124001, India.
Biotechnol Lett. 2017 Oct;39(10):1453-1462. doi: 10.1007/s10529-017-2396-0. Epub 2017 Jul 19.
Bilirubin detection plays a major role in healthcare. Its high concentration in human serum is lethal and must be determined accurately. Clinically, it is vital for assessing patients with deleterious health conditions such as jaundice or icterus, hepatitis, mental disorders, cerebral palsy and brain damage especially in the case of neonates. In evaluating the drawbacks regarding the conventional methodology of bilirubin detection, there is need for a superior analytical tool. Bilirubin oxidase (BOx)-based sensors have been designed for the ultrasensitive analysis of bilirubin and quality deliverance of treatment and this review highlights the different mechanisms of bilirubin detection using different modified electrodes. Further, it also addresses the exploitation of highly attractive electrocatalytic properties of elite nanoparticles such as gold and zirconia- coated silica nanoparticles in enhancing the reproducibility and specificity of bilirubin biosensors.
胆红素检测在医疗保健中起着重要作用。其在人体血清中的高浓度是致命的，必须准确测定。临床上，对于评估患有黄疸或黄疸、肝炎、精神障碍、脑瘫和脑损伤等有害健康状况的患者至关重要，尤其是在新生儿中。在评估传统胆红素检测方法的缺点时，需要一种更高级的分析工具。基于胆红素氧化酶（BOx）的传感器已被设计用于胆红素的超灵敏分析和治疗质量的提供，本综述重点介绍了使用不同修饰电极检测胆红素的不同机制。此外，它还探讨了金和氧化锆包覆二氧化硅纳米颗粒等优质纳米颗粒极具吸引力的电催化特性在提高胆红素生物传感器的重现性和特异性方面的应用。
