Yang Wei, Xia Jinfeng, Zhou Guohong, Jiang Danyu, Li Qiang
Department of Chemistry, East China Normal University Shanghai 200062 P. R. China
Shanghai Institute of Ceramics, Chinese Academy of Sciences Shanghai 200050 P. R. China.
RSC Adv. 2018 May 16;8(32):17854-17859. doi: 10.1039/c8ra02817f. eCollection 2018 May 14.
Free bilirubin, when present in excess in the human body, can cause a multitude of diseases and disorders and even be fatal; hence, detecting it is of paramount importance. Herein, we report a luminescence quenching-based non-enzymatic method for the convenient, reliable, and rapid detection of free bilirubin in blood serum samples using sensing films (nanosheets/PS, nanosheets-tta/PS, and nanosheets-dbt/PS) as luminescent sensors. The luminescence intensity of the sensing films is linearly related to the free bilirubin concentration. Nanosheets-tta/PS demonstrated excellent sensing properties for the sensitive and reliable detection of free bilirubin in the range of 0.0-60.0 μM with a correlation coefficient of 0.9915, as compared to nanosheets/PS or nanosheets-dbt/PS. The limit of detection for the determination of free bilirubin was 41 nM. This method can be used to design a sensor-based test spot as a medical detection device for the visual detection of free bilirubin.
当人体内游离胆红素过量时，会引发多种疾病和功能紊乱，甚至危及生命；因此，对其进行检测至关重要。在此，我们报告了一种基于发光猝灭的非酶法，该方法使用传感膜（纳米片/聚苯乙烯、纳米片-tta/聚苯乙烯和纳米片-dbt/聚苯乙烯）作为发光传感器，可方便、可靠且快速地检测血清样本中的游离胆红素。传感膜的发光强度与游离胆红素浓度呈线性关系。与纳米片/聚苯乙烯或纳米片-dbt/聚苯乙烯相比，纳米片-tta/聚苯乙烯在0.0 - 60.0 μM范围内对游离胆红素具有出色的传感性能，可实现灵敏可靠的检测，相关系数为0.9915。游离胆红素测定的检测限为41 nM。该方法可用于设计基于传感器的测试点，作为用于目视检测游离胆红素的医学检测设备。
