Faculty of Science, Minia University, Minia 61519, Egypt.
Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan; Research Center for Animal Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
Cytokine. 2017 Nov;99:66-72. doi: 10.1016/j.cyto.2017.07.008. Epub 2017 Jul 17.
The current study aimed to confirm and examine the physiological roles of the proinflammatory cytokines IL1B and IL6 on the immune functions which mediated by cathelicidins (CATHs) in the uterus and vagina of laying hens. Snaps of the mucosal tissues of uterus and vagina were incubated in culture medium or chicken recombinant IL1B and IL6 for 1.5h or 3h before extraction of total RNA to be used for examination of IL1B and IL6, their receptors, and cathelicidins by semi-quantitative PCR; and to examine the changes in cathelicidins expressions by real-time PCR. PCR analysis confirmed that IL1B and IL6, their receptors, and CATH1-3 were expressed in the mucosal tissues of the uterus and vagina. In uterus tissue, IL1B did not affect the expression of CATH1 and -2 at different doses and incubation time, whereas CATH3 was significantly downregulated by incubation with IL1B for 1.5h. In the vaginal tissue, the expressed CATH1, -2 and -3 were significantly upregulated by incubation with IL1B for 1.5h in a dose-dependent manner. In uterus tissue, CATH1 expression was down-regulate by IL6 incubation for 1.5h, but not by 3h however, CATH3 expression was significantly increased by incubation with IL6 for 1.5h, but not for 3h. In the vaginal tissues, all CATHs expression was not affected significantly by incubation with IL6. These current observations suggest that CATH1, -2 and -3 in the vagina are upregulated by IL1B, and CATH3 in the uterus is also upregulated by IL6. IL1B and IL6 synthesized in response to infection by the microbes may enhance the defense system in the oviduct mucosal tissues by increasing the synthesis of CATHs.
本研究旨在确认并研究促炎细胞因子白细胞介素 1B（IL1B）和白细胞介素 6（IL6）在蛋鸡子宫和阴道中通过抗菌肽（CATHs）介导的免疫功能中的生理作用。在提取总 RNA 之前，将子宫和阴道的黏膜组织片段在培养基或鸡重组白细胞介素 1B 和白细胞介素 6 中孵育 1.5 或 3 小时，然后通过半定量 PCR 检测白细胞介素 1B 和白细胞介素 6、其受体和抗菌肽；并通过实时 PCR 检测抗菌肽表达的变化。PCR 分析证实，白细胞介素 1B 和白细胞介素 6、其受体和 CATH1-3 均在子宫和阴道的黏膜组织中表达。在子宫组织中，白细胞介素 1B 以剂量和孵育时间依赖的方式，在不同剂量和孵育时间下均不影响 CATH1 和 -2 的表达，而 CATH3 则被白细胞介素 1B 孵育 1.5 小时显著下调。在阴道组织中，CATH1、-2 和 -3 的表达在孵育 1.5 小时后，均被白细胞介素 1B 以剂量依赖的方式显著上调。在子宫组织中，CATH1 的表达在白细胞介素 6 孵育 1.5 小时后被下调，但在 3 小时后则不受影响；然而，CATH3 的表达在白细胞介素 6 孵育 1.5 小时后显著增加，但在 3 小时后则不受影响。在阴道组织中，白细胞介素 6 孵育对所有 CATH 表达均无显著影响。这些研究结果表明，阴道中的 CATH1、-2 和 -3 被白细胞介素 1B 上调，而子宫中的 CATH3 也被白细胞介素 6 上调。对微生物感染作出反应而合成的白细胞介素 1B 和白细胞介素 6 可能通过增加抗菌肽的合成来增强输卵管黏膜组织的防御系统。
