Chen Lu, Yang Maosen, Zhu Wei, Su Yuan, Li Diyan, Wang Tao
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
School of Pharmacy, Chengdu University, Chengdu, China.
Front Microbiol. 2022 Feb 16;13:846011. doi: 10.3389/fmicb.2022.846011. eCollection 2022.
The reproductive tract of chickens is an important organ for egg formation. The vagina is in close contact with the external environment, which may lead to the invasion of a variety of pathogenic bacteria, affect the internal and external quality of eggs, and even increase mortality and cause economic loss. In recent years, probiotics as a substitute for antibiotics have brought economic benefits in livestock and poultry production. In the present study, we investigated the effects of vaginal administration of on the cloacal microbiota, vaginal transcriptome and metabolomics of chickens and evaluated the beneficial potential of The results showed that treatment could affect the microbial composition of the cloaca. Transcriptome analysis found that the immune-related genes , , and were upregulated, that the inflammatory genes , , and were downregulated, and that DEGs were also enriched in the regulation of the inflammatory response, cellular metabolism, and synaptic response pathways. In addition, the differential metabolites were mainly related to steroid hormone biosynthesis, unsaturated fatty acid biosynthesis, and arachidonic acid metabolism, and we identified associations between specific differential metabolites and genes. Overall, this study provides a theoretical basis for the application of as a potential probiotic in livestock and poultry production.
鸡的生殖道是形成鸡蛋的重要器官。阴道与外部环境密切接触，这可能导致多种病原菌入侵，影响鸡蛋的内外品质，甚至增加死亡率并造成经济损失。近年来，益生菌作为抗生素的替代品，在畜禽生产中带来了经济效益。在本研究中，我们研究了阴道给药[未提及具体物质]对鸡泄殖腔微生物群、阴道转录组和代谢组的影响，并评估了[未提及具体物质]的有益潜力。结果表明，[未提及具体物质]处理可影响泄殖腔的微生物组成。转录组分析发现，免疫相关基因[未提及具体基因]、[未提及具体基因]和[未提及具体基因]上调，炎症相关基因[未提及具体基因]、[未提及具体基因]和[未提及具体基因]下调，差异表达基因也富集在炎症反应、细胞代谢和突触反应途径的调控中。此外，差异代谢物主要与类固醇激素生物合成、不饱和脂肪酸生物合成和花生四烯酸代谢有关，我们还确定了特定差异代谢物与基因之间的关联。总体而言，本研究为[未提及具体物质]作为潜在益生菌在畜禽生产中的应用提供了理论依据。
