National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of the Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.
Key Laboratory of Silviculture and Conservation of the Ministry of Education, College of Forestry, Beijing Forestry University, Beijing, 100083, China.
Sci Rep. 2017 Jul 20;7(1):6048. doi: 10.1038/s41598-017-06255-3.
Pollination is an important event in plant sexual reproduction, and post-pollination response is an essential process for reproduction. Populus alba × P. glandulosa is used widely in scientific research, especially in cross breeding as parents. Adult female P. alba × P. glandulosa flowers are highly compatible with pollen from male P. tomentosa, but the early post-pollination response of flowers at the molecular levels is unclear. In this study, RNA-seq was employed to comprehensively understand the response of female P. alba × P. glandulosa flowers to pollination. Enrichment analysis reveals that the 'plant hormone signal transduction' pathway is enhanced during pollen-pistil interaction. Moreover, genes related to auxin, gibberellin and ethylene biosynthesis were significantly up-regulated. Ca and H-related genes and cell wall-related genes are interrelated, and all of them are essential for pollen tube elongation in pistil, especially, free Ca providing a concentration gradient for pollen tube guidance and involved in signal transduction. Furthermore, RNA-seq results indicate that genes involved in the adhesion and guidance for pollen germination and pollen tube growth are abundantly present in the extracellular matrix. Our study provides an overview and detailed information for understanding the molecular mechanism of early post-pollination response in this hybrid poplar reproduction.
授粉是植物有性生殖中的一个重要事件，而授粉后反应是生殖的一个重要过程。银白杨 × 腺毛杨被广泛应用于科学研究，特别是作为杂交亲本进行杂交。成年雌性银白杨 × 腺毛杨花朵与雄性毛白杨花粉高度兼容，但花朵在分子水平上的早期授粉后反应尚不清楚。在这项研究中，使用 RNA 测序全面了解雌性银白杨 × 腺毛杨花朵对授粉的反应。富集分析表明，在花粉-雌蕊相互作用过程中，“植物激素信号转导”途径得到增强。此外，与生长素、赤霉素和乙烯生物合成相关的基因显著上调。钙和 H 相关基因与细胞壁相关基因相互关联，它们都是雌蕊中花粉管伸长所必需的，特别是游离钙为花粉管导向提供浓度梯度，并参与信号转导。此外，RNA 测序结果表明，参与花粉萌发和花粉管生长的粘附和导向的基因在细胞外基质中大量存在。本研究为理解这种杂交杨繁殖中早期授粉后反应的分子机制提供了一个概述和详细信息。
