Yang Mengdi, Yarra Rajesh, Zhang Ruining, Zhou Lixia, Jin Longfei, Martin Jerome Jeyakumar John, Cao Hongxing
Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, Hainan, 571339, People's Republic of China.
Hainan Key Laboratory of Tropical Oil Crops Biology, Wenchang, Hainan, 571339, People's Republic of China.
Funct Integr Genomics. 2022 Apr;22(2):261-278. doi: 10.1007/s10142-022-00834-y. Epub 2022 Feb 28.
Phytohormones play an important role in the pollination and fertilization of crops, but the regulatory mechanisms of oil palm pollination and fertilization are unclear. The purpose of this study is to explore the hormonal changes of oil palm pistils during flowering. We used RNA sequencing to evaluate differentially expressed genes (DEGs) in oil palm pistils at the pollination and non-pollination stages. In this study, we found that the hormone contents of oil palm pistil changed drastically after pollination. The transcriptome of the oil palm pistil without pollination and at 2 h, 4 h, 12 h, 24 h, and 48 h after pollination was comprehensively analyzed, and a large number of differential genes and metabolic pathways were explored. Based on the transcriptome data, it could be recognized that the changes of indoleacetic acid (IAA), zeatin riboside (ZR), and abscisic acid (ABA) during pollination were consistent with the changes in the corresponding gene transcripts. Differentially expressed genes during pollination and fertilization of oil palm were mainly related to energy metabolism and hormone signal transduction. It provides new insights to elucidate the interaction and regulation mechanisms of plant hormones before and after oil palm pollination, providing a theoretical basis and reference for the research on sexual reproduction of oil palm.
植物激素在作物授粉和受精过程中发挥着重要作用，但油棕授粉和受精的调控机制尚不清楚。本研究的目的是探索油棕雌蕊在开花期间的激素变化。我们使用RNA测序来评估油棕雌蕊在授粉和未授粉阶段的差异表达基因（DEG）。在本研究中，我们发现油棕雌蕊授粉后激素含量发生了剧烈变化。对未授粉以及授粉后2小时、4小时、12小时、24小时和48小时的油棕雌蕊转录组进行了综合分析，探索了大量差异基因和代谢途径。基于转录组数据，可以认识到授粉期间吲哚乙酸（IAA）、玉米素核苷（ZR）和脱落酸（ABA）的变化与相应基因转录本的变化一致。油棕授粉和受精过程中的差异表达基因主要与能量代谢和激素信号转导有关。这为阐明油棕授粉前后植物激素的相互作用和调控机制提供了新的见解，为油棕有性生殖的研究提供了理论依据和参考。
