Kaposvár University, Faculty of Agricultural and Environmental Sciences, Kaposvár, Hungary.
Kaposvár University, Faculty of Agricultural and Environmental Sciences, Kaposvár, Hungary </br>MTA-KE Mycotoxins in the Food Chain Research Group, Kaposvár University, Kaposvár, Hungary.
Pol J Microbiol. 2017 Jul 6;66(2):245-250. doi: 10.5604/01.3001.0010.7858.
The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B1 (5 μg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for enumerating the amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in the amount of bacterial groups between the experimental (buffer, chyme, and fumonisin B) and control 1 groups (buffer + chyme) were observed in both methods. Fumonisin B and hydrolysed fumonisin B concentration were analysed by liquid chromatograghy - mass spectrometry. There was no significant difference in FB concentration between the experimental and the control 2 group (buffer and fumonisin B) at 0 h incubation, 5.185 ± 0.174 μg/ml compared with 6.433 ± 0.076 μg/ml. Fumonisin B concentration in the experimental group was reduced to 4.080 ± 0.065 μg/ml at 24 h and to 2.747 ± 0.548 μg/ml at 48 h incubation and was significantly less than that of in the control group. Hydrolysed fumonisin B was detected after 24 h incubation (0.012 ± 0 μg/ml). At 48 h incubation time, hydrolysed fumonisin B concentration was doubled to 0.024 ± 0.004 μg/ml. These results indicate that fumonisin B can be metabolised by caecal microbiota in pigs though the number of studied bacteria did not change.
将猪的盲肠内容物在 McDougall 缓冲液中进行厌氧孵育，分别添加和不添加伏马菌素 B1（5μg/ml），孵育 0、24 和 48 小时。采用平板计数琼脂技术对包括需氧菌、厌氧菌、大肠菌群、大肠杆菌和乳酸杆菌在内的细菌数量进行计数。还进行了定量聚合酶链反应，以估计总细菌、乳酸杆菌、拟杆菌和普雷沃氏菌的数量。在这两种方法中，实验组（缓冲液、食糜和伏马菌素 B）和对照组 1 组（缓冲液+食糜）之间细菌数量的细菌群没有显著差异。采用液相色谱-质谱法分析伏马菌素 B 和水解伏马菌素 B 的浓度。在 0 小时孵育时，实验组和对照组 2 组（缓冲液和伏马菌素 B）之间 FB 浓度没有差异，分别为 5.185±0.174μg/ml 和 6.433±0.076μg/ml。在 24 小时孵育时，实验组中的伏马菌素 B 浓度降低至 4.080±0.065μg/ml，48 小时孵育时降低至 2.747±0.548μg/ml，明显低于对照组。24 小时孵育后检测到水解伏马菌素 B（0.012±0μg/ml）。在 48 小时孵育时间时，水解伏马菌素 B 的浓度增加到 0.024±0.004μg/ml。这些结果表明，伏马菌素 B 可通过猪盲肠微生物群代谢，尽管研究的细菌数量没有变化。
