GenPhySE, Université de Toulouse, INRA, INPT, ENVT, 31326 Castanet-Tolosan, France.
Lallemand SAS, 19 rue des Briquetiers, BP 59, 31702 Blagnac CEDEX, France.
Toxins (Basel). 2018 Jun 5;10(6):230. doi: 10.3390/toxins10060230.
Pigs are highly affected by dietary mycotoxin contamination and particularly by fumonisin. The effects of fumonisin on pig intestinal health are well documented, but little is known regarding its impact on gut microbiota. We investigate the effects of the fumonisin (FB1, 12 mg/kg feed) on the fecal microbiota of piglets ( = 6) after 0, 8, 15, 22, and 29 days of exposure. A control group of six piglets received a diet free of FB1. Bacterial community diversity, structure and taxonomic composition were carried out by V3⁻V4 16S rRNA gene sequencing. Exposure to FB1 decreases the diversity index, and shifts and constrains the structure and the composition of the bacterial community. This takes place as early as after 15 days of exposure and is at a maximum after 22 days of exposure. Compared to control, FB1 alters the ecological succession of fecal microbiota species toward higher levels of and lower levels of the Lachnospiraceae and Veillonellaceae families, and particularly OTUs (Operational Taxonomic Units) of the genera , and . In conclusion, FB1 shifts and constrains age-related evolution of microbiota. The direct or indirect contribution of FB1 microbiota alteration in the global host response to FB1 toxicity remains to be investigated.
猪对日粮中霉菌毒素污染非常敏感，尤其是伏马菌素。伏马菌素对猪肠道健康的影响已有充分的文献记载，但对其对肠道微生物群的影响知之甚少。我们研究了在暴露 0、8、15、22 和 29 天后，12mg/kg 饲料的伏马菌素（FB1）对仔猪粪便微生物群的影响（每组 = 6）。一组 6 只仔猪接受不含 FB1 的饮食。通过 V3-V4 16S rRNA 基因测序进行细菌群落多样性、结构和分类组成分析。暴露于 FB1 会降低多样性指数，并改变和限制细菌群落的结构和组成。这种情况早在暴露 15 天后就出现了，在暴露 22 天后达到最大值。与对照组相比，FB1 改变了粪便微生物群物种的生态演替，使水平上升，而水平下降，Lachnospiraceae 和 Veillonellaceae 家族以及特别是属的 OTUs（操作分类单元）、和 。总之，FB1 改变和限制了与年龄相关的微生物群进化。FB1 对微生物群的直接或间接改变在宿主对 FB1 毒性的整体反应中的作用仍有待研究。
