Caloni F, Spotti M, Auerbach H, Op den Camp H, Gremmels J F, Pompa G
Facoltà di Medicina Veterinaria, Istituto di Farmacologia e Tossicologia Veterinaria, Milan, Italy.
Vet Res Commun. 2000 Sep;24(6):379-87. doi: 10.1023/a:1006422200226.
Fumonisin B1 (FB1) is a mycotoxin produced by Fusarium moniliforme and F. proliferatum. Little is known of its metabolic fate after oral ingestion in ruminants, but these animals are reported to be tolerant towards FB1. The metabolism of this mycotoxin was evaluated following incubation (1 microg/ml) in ruminal fluid for up to 72 h, in the presence or absence of alfalfa as a substrate for microbial growth, using a model rumen (sealed flask, anaerobic conditions, exclusion of light, gentle agitation, 39 degrees C). The decrease in FB1 concentration and the production of short-chain fatty acids were determined. FB1 had no effect on SCFA production. After 72 h incubation, FB1 depletion was 12% and 18% in samples with and without alfalfa, respectively. No hydrolysed metabolites (aminopolyols or aminopentol) were detected. These results indicate that FB1 is poorly metabolized in the rumen and suggest that such metabolism is not the cause of the tolerance to this toxin displayed by ruminants.
伏马菌素B1（FB1）是由串珠镰刀菌和层出镰刀菌产生的一种霉菌毒素。关于反刍动物口服后它的代谢归宿知之甚少，但据报道这些动物对FB1具有耐受性。使用模拟瘤胃（密封烧瓶，厌氧条件，避光，轻轻搅拌，39℃），在有或没有苜蓿作为微生物生长底物的情况下，在瘤胃液中以1微克/毫升的浓度孵育长达72小时，评估这种霉菌毒素的代谢情况。测定了FB1浓度的降低和短链脂肪酸的产生。FB1对短链脂肪酸的产生没有影响。孵育72小时后，有苜蓿和没有苜蓿的样品中FB1的消耗分别为12%和18%。未检测到水解代谢产物（氨基多元醇或氨基戊醇）。这些结果表明FB1在瘤胃中代谢不良，并表明这种代谢不是反刍动物对这种毒素耐受性的原因。
