Kaundal Nirmal, Sarkate Purva, Prakash Charu, Rishi Narayan
Virology-1 Laboratory, Microbiology Division, National Centre for Disease Control, 22-Shamnath Marg, Civil Lines, Delhi 110054 India.
Microbiology Division, National Centre for Disease Control, 22-Shamnath Marg, Civil Lines, Delhi 110054 India.
Virusdisease. 2017 Dec;28(4):383-389. doi: 10.1007/s13337-017-0409-1. Epub 2017 Nov 28.
With the eradication of poliovirus, the focus has now shifted to environmental surveillance of poliovirus to determine the circulating polioviruses in an area. L20B and RD cell lines are used for isolation of polioviruses. It is imperative to study the efficacy of these cell line in isolating polioviruses from environmental samples. The present study was carried out to determine the sensitivity and specificity of L20B cell line for isolation of polioviruses from environmental samples. L20B and RD cell lines are used for isolation of polioviruses. Molecular characterization was done by using real time RT-PCR. A total of 432 sewage samples from Delhi and Punjab were processed for the isolation of polioviruses during Jan-Dec 2015. 96.76% of the samples were positive in either of the cell lines. Non-polio enteroviruses were obtained in 50 samples on primary isolation. On RT-PCR, 347 (94.29%) samples yielded polioviruses and the rest (21) non-polio enteroviruses or non-enteroviruses. A total of 703 isolates were obtained. 635 isolates were found polioviruses by PCR (90.33%), 20 isolates were found to be NPEV (2.84%) and 48 (6.83%) were found to be NEV. Out of the 20 NPEV isolates, 14 were from RLR (RD-L20B-RD) route and six isolates were from LR (L20B-RD) route. All 48 NEV isolates were from LR route. Thus L20B cell line is more sensitive as compared to RD cell line for isolation of polioviruses however it is not absolutely specific for polioviruses.
随着脊髓灰质炎病毒的根除，目前重点已转向脊髓灰质炎病毒的环境监测，以确定某一地区流行的脊髓灰质炎病毒。L20B和RD细胞系用于脊髓灰质炎病毒的分离。研究这些细胞系从环境样本中分离脊髓灰质炎病毒的效力至关重要。本研究旨在确定L20B细胞系从环境样本中分离脊髓灰质炎病毒的敏感性和特异性。L20B和RD细胞系用于脊髓灰质炎病毒的分离。通过实时逆转录聚合酶链反应进行分子特征分析。2015年1月至12月期间，共处理了来自德里和旁遮普邦的432份污水样本以分离脊髓灰质炎病毒。96.76%的样本在任一细胞系中呈阳性。初次分离时在50份样本中获得了非脊髓灰质炎肠道病毒。逆转录聚合酶链反应检测时，347份（94.29%）样本检出脊髓灰质炎病毒，其余21份样本为非脊髓灰质炎肠道病毒或非肠道病毒。共获得703株分离株。通过聚合酶链反应发现635株分离株为脊髓灰质炎病毒（90.33%），20株为非脊髓灰质炎肠道病毒（2.84%），48株为非肠道病毒（6.83%）。在20株非脊髓灰质炎肠道病毒分离株中，14株来自RLR（RD-L20B-RD）途径，6株来自LR（L20B-RD）途径。所有48株非肠道病毒分离株均来自LR途径。因此，与RD细胞系相比，L20B细胞系在分离脊髓灰质炎病毒方面更敏感，然而它对脊髓灰质炎病毒并非绝对特异。
