Braga Juliana Fortes Vilarinho, Leal Carlos Augusto Gomes, Silva Camila Costa, Fernandes André Almeida, Martins Nelson Rodrigo da Silva, Ecco Roselene
a Departamento de Clínica e Cirurgia Veterinária , Escola de Veterinária, Universidade Federal de Minas Gerais , Belo Horizonte , Brazil.
b Departamento de Medicina Veterinária Preventiva , Escola de Veterinária, Universidade Federal de Minas Gerais , Belo Horizonte , Brazil.
Avian Pathol. 2018 Feb;47(1):14-22. doi: 10.1080/03079457.2017.1359403. Epub 2017 Aug 24.
Vertebral osteomyelitis (VO) is a worldwide emerging disease that affects broilers. Recently, the isolation of Enterococcus faecalis in cases of the disease has been described. This study aimed at determining the genetic diversity and antimicrobial resistance profile of 12 E. faecalis strains isolated from broilers with VO. Strains were isolated from nine flocks from six farms in a high-density poultry production area in Southeast Brazil and were evaluated using multilocus sequence typing and phylogenetic analysis. Antimicrobial susceptibility tests and PCR were performed to detect antimicrobial resistance genes. E. faecalis isolates belonged to different sequence types (ST), six of which (ST49, ST100, ST116, ST202, ST249, and ST300) have been previously described. Strains ST708 and ST709 were newly identified in this study. Strain ST49 was most frequently isolated (50% of the flocks) from the analysed VO cases. No phylogenetic or phylogeographic relationship was found among the strains. The VO isolated E. faecalis strains showed highest resistance to aminoglycosides, mainly gentamicin (40%), but were highly susceptible to vancomycin (10%). Aminoglycoside resistance genes were detected in seven E. faecalis strains, and AAC6'-APH2″ genes were most frequently detected. The results showed that E. faecalis strains isolated from recently reported VO cases were highly diverse genetically. The diversity of genotypes in circulation in the analysed flocks, without apparent relationship among them, raises questions on aetiopathogenesis of the disease in broilers and evolutionary aspects of E. faecalis.
脊椎骨髓炎（VO）是一种影响肉鸡的全球性新兴疾病。最近，已有关于在该疾病病例中分离出粪肠球菌的报道。本研究旨在确定从患有VO的肉鸡中分离出的12株粪肠球菌菌株的遗传多样性和抗菌药物耐药性谱。这些菌株是从巴西东南部一个高密度家禽生产区的六个农场的九个鸡群中分离出来的，并使用多位点序列分型和系统发育分析进行评估。进行了抗菌药物敏感性试验和聚合酶链反应（PCR）以检测抗菌药物耐药基因。粪肠球菌分离株属于不同的序列类型（ST），其中六种（ST49、ST100、ST116、ST202、ST249和ST300）先前已有描述。ST708和ST709菌株是本研究中新鉴定出的。ST49菌株在分析的VO病例中分离频率最高（占鸡群的50%）。在这些菌株之间未发现系统发育或系统地理学关系。从VO分离出的粪肠球菌菌株对氨基糖苷类药物耐药性最高，主要是庆大霉素（40%），但对万古霉素高度敏感（10%）。在七株粪肠球菌菌株中检测到了氨基糖苷类耐药基因，并最常检测到AAC6'-APH2″基因。结果表明，从最近报道的VO病例中分离出的粪肠球菌菌株在遗传上高度多样。分析鸡群中循环的基因型多样性，且它们之间无明显关系，这对肉鸡该疾病的病因发病机制以及粪肠球菌的进化方面提出了疑问。
