用于产气荚膜梭菌和奇异梭菌的改良质粒分离方法。
Modified plasmid isolation method for Clostridium perfringens and Clostridium absonum.
作者信息
Roberts I, Holmes W M, Hylemon P B
出版信息
Appl Environ Microbiol. 1986 Jul;52(1):197-9. doi: 10.1128/aem.52.1.197-199.1986.
Abstract
A rapid plasmid isolation procedure for Clostridium perfringens and C. absonum is described. The ratio of culture volume to lysis buffer volume was found to be crucial for efficient plasmid isolation. The method can be scaled up, without difficulty, for large-scale plasmid preparation.
摘要
本文描述了一种用于产气荚膜梭菌和奇异梭菌的快速质粒分离方法。发现培养物体积与裂解缓冲液体积的比例对于高效质粒分离至关重要。该方法可以毫无困难地扩大规模用于大规模质粒制备。
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