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用于动物生产的细胞遗传学检测:现状与展望

Cytogenetic tests for animal production: state of the art and perspectives.

作者信息

Udroiu I, Sgura A

机构信息

Dipartimento di Scienze, Università Roma Tre, Viale G. Marconi 446, 00146, Rome, Italy.

出版信息

Anim Genet. 2017 Oct;48(5):505-515. doi: 10.1111/age.12581. Epub 2017 Jul 25.

Abstract

Cytogenetic tests are effective tools for monitoring the health status of livestock and improving their genetic value. Cytogenetic screening allows for the detection of animals carrying chromosomal aberrations and to avoid using them as breeders. Progress in karyotype monitoring, with new molecular probes and automation, has greatly increased the productivity of this procedure. Several genotoxicity tests are available to detect the possible presence and effects of pollutants or drugs. Among these, the micronucleus test and the Comet assay are the most convenient in terms of costs and benefits. Finally, analysis of telomeres, the end of chromosomes and markers of genomic instability, may be developed into a new marker of stress and genetic value.

摘要

细胞遗传学检测是监测家畜健康状况和提高其遗传价值的有效工具。细胞遗传学筛查能够检测出携带染色体畸变的动物,并避免将它们用作种畜。随着新的分子探针和自动化技术的出现,核型监测取得了进展,极大地提高了这一程序的效率。有几种遗传毒性检测方法可用于检测污染物或药物的可能存在及其影响。其中,微核试验和彗星试验在成本效益方面最为便捷。最后,对端粒(染色体末端和基因组不稳定性的标志物)的分析可能会发展成为应激和遗传价值的新标志物。

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