College of Forestry, Northwest A&F University, Yangling 712100, China.
Tianjin Forestry Pest Control and Quarantine Station, Tianjin 300000, China.
Int J Mol Sci. 2018 Jul 10;19(7):2010. doi: 10.3390/ijms19072010.
We comprehensively investigated the biodiversity of fungal communities in different developmental stages of and the difference between sexes and two generations by high throughput sequencing. The predominant species found in the intestinal fungal communities mainly belong to the phyla Ascomycota and Basidiomycota. Fungal community structure varies with life stage. The genera Nakazawaea, Trichothecium, Aspergillus, Didymella, Villophora, and Auricularia are most prevalent in the larvae samples. Adults harbored high proportions of Graphium. The fungal community structures found in different sexes are similar. Fusarium is the most abundant genus and conserved in all development stages. Gut fungal communities showed notable variation in relative abundance during the overwintering stage. Fusarium and Nectriaceae were significantly increased in overwintering mature larvae. The data indicates that Fusarium might play important roles in the survival of especially in the overwintering stage. The authors speculated that plays an important role in the invasion and colonization of . The study will contribute to the understanding of the biological role of the intestinal fungi in , which might provide an opportunity and theoretical basis to promote integrated pest management (IPM) of .
我们通过高通量测序全面研究了不同发育阶段和雌雄两性及两代 肠道真菌群落的生物多样性。在肠道真菌群落中发现的主要优势种属于子囊菌门和担子菌门。真菌群落结构随生活阶段而变化。在幼虫样本中，最常见的属为 Nakazawaea、 Trichothecium、Aspergillus、Didymella、Villophora 和 Auricularia。成虫中 Graphium 的比例较高。不同性别之间的真菌群落结构相似。在所有发育阶段，最丰富的属是 Fusarium，且保持不变。在越冬阶段，肠道真菌群落的相对丰度表现出明显的变化。在越冬成熟幼虫中， Fusarium 和 Nectriaceae 的丰度显著增加。数据表明， Fusarium 可能在 的生存中发挥重要作用，尤其是在越冬阶段。作者推测 在 的入侵和定植中发挥重要作用。本研究将有助于了解肠道真菌在 中的生物学作用，这可能为促进 的综合虫害管理（IPM）提供机会和理论基础。
