噬菌体溶菌酶及其应用。
Bacteriophage endolysins and their applications.
作者信息
Ajuebor Jude, McAuliffe Olivia, O'Mahony Jim, Ross R Paul, Hill Colin, Coffey Aidan
出版信息
Sci Prog. 2016 Jun 1;99(2):183-199. doi: 10.3184/003685016X14627913637705.
Abstract
Endolysins (lysins) are bacteriophage-encoded enzymes that have evolved to degrade specific bonds within the bacterial cell wall. These enzymes represent a novel class of antibacterial agents against infectious pathogens, especially in light of multidrug-resistant bacteria, which have made antibiotic therapy increasingly redundant. Lysins have been used successfully to eliminate/control bacterial pathogens in various anatomical locations in mouse and other animal models. Engineering tactics have also been successfully applied to improve lysin function. This review discusses the structure and function of lysins. It highlights protein-engineering tactics utilised to improve lysin activity. It also reviews the applications of lysins towards food biopreservation, therapeutics, biofilm elimination and diagnostics.
摘要
内溶素（溶素）是噬菌体编码的酶，其进化目的是降解细菌细胞壁内的特定化学键。这些酶代表了一类新型的抗传染病病原体抗菌剂，尤其是鉴于多重耐药细菌的出现，使得抗生素治疗越来越无用。溶素已成功用于消除/控制小鼠和其他动物模型中各种解剖部位的细菌病原体。工程策略也已成功应用于改善溶素功能。本综述讨论了溶素的结构和功能。它重点介绍了用于提高溶素活性的蛋白质工程策略。它还综述了溶素在食品生物保鲜、治疗、生物膜消除和诊断方面的应用。
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2
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Viruses. 2015 Jun 12;7(6):3019-34. doi: 10.3390/v7062758.
3
A novel and highly specific phage endolysin cell wall binding domain for detection of Bacillus cereus.一种用于检测蜡样芽孢杆菌的新型且高度特异性的噬菌体溶菌酶细胞壁结合结构域。
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