College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, PR China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, PR China.
College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, PR China; Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, PR China.
Biosens Bioelectron. 2018 Jan 15;99:92-98. doi: 10.1016/j.bios.2017.07.050. Epub 2017 Jul 20.
A novel and competitive electrochemiluminescence (ECL) aptasensor for prostate specific antigen (PSA) assay was constructed using gold nanorods functionalized graphene oxide (GO@AuNRs) multilabeled with glucose oxidase (GOD) and streptavidin (SA) toward luminol-based ECL system. A strong initial ECL signal was achieved by electrodeposited gold (DpAu) on the electrode because of gold nanoparticles (AuNPs) motivating the luminol ECL signal. The signal probes prepared by loading GOD and SA-biotin-DNA on GO@AuNRs were used for achieving multiple signal amplification. In the absence of PSA, the signal probes can be attached on the electrode by hybridization reaction between PSA aptamer and biotin-DNA. In this state, the GOD loaded on the probe could catalyze glucose to in situ produce HO and then AuNRs catalyze HO to generate abundant reactive oxygen species (ROSs) in luminol ECL reaction. Both the high-content GOD and AuNRs in the signal probe amplified the ECL signal in the ECL system. Moreover, the combination of SA with biotin-DNA further expands ECL intensity. The integration of such amplifying effects in this protocol endows the aptasensor with high sensitivity and good selectivity for PSA detection. This aptasensor exhibits a linear relation in the range of 0.5pgmL to 5.0ngmL with the detection limit of 0.17pgmL (S/N = 3). Besides, the strategy was successfully applied in determination of human serum samples with recovery of 81.4-116.0%.
一种新颖且具有竞争力的电化学发光（ECL）适体传感器，用于前列腺特异性抗原（PSA）检测，是使用金纳米棒功能化氧化石墨烯（GO@AuNRs）构建的，该纳米棒通过葡萄糖氧化酶（GOD）和链霉亲和素（SA）多标记，针对鲁米诺的 ECL 系统。由于金纳米粒子（AuNPs）激发了鲁米诺的 ECL 信号，因此通过在电极上电沉积金（DpAu）实现了初始强 ECL 信号。通过将 GOD 和 SA-生物素-DNA 加载到 GO@AuNRs 上制备信号探针，以实现多重信号放大。在不存在 PSA 的情况下，信号探针可以通过 PSA 适体与生物素-DNA 之间的杂交反应附着在电极上。在此状态下，加载在探针上的 GOD 可以催化葡萄糖原位产生 HO，然后 AuNRs 催化 HO 在鲁米诺 ECL 反应中产生大量活性氧物种（ROSs）。信号探针中高含量的 GOD 和 AuNRs 均在 ECL 系统中放大了 ECL 信号。此外，SA 与生物素-DNA 的结合进一步扩展了 ECL 强度。这种方案中各种放大效果的整合赋予了适体传感器对 PSA 检测的高灵敏度和良好选择性。该适体传感器在 0.5pgmL 至 5.0ngmL 的范围内呈现线性关系，检测限为 0.17pgmL（S/N = 3）。此外，该策略成功应用于人血清样品的测定，回收率为 81.4-116.0%。
