Stomatological Hospital of Chongqing Medical University, Chongqing, 401147, China.
Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Municipal Key, Chongqing, 401147, China.
Sci Rep. 2017 Jul 25;7(1):6373. doi: 10.1038/s41598-017-06548-7.
Cells, scaffolds, and growth factors play important roles in bone regeneration. Bone morphogenetic protein 9 (BMP9), a member of BMP family, could facilitate osteogenesis by regulating growth factors and promoting angiogenesis. Similar to other stem cells, rat dental follicle stem cells (rDFCs), the precursor cells of cementoblasts, osteoblasts and periodontal ligament cells, can self-renew and exhibit multipotential capacity. Coralline hydroxyapatite (CHA) has good biocompatibility and conductivity required for bone tissue engineering. Here, we reported that BMP9 could enhance the osteogenic differentiation of rDFCs in cell culture. Moreover, our results suggested that BMP9 acted through the Smad1/5/8 signaling pathway. We also produced a novel scaffold that encompasses bio-degradable CHA seeded with recombinant adenoviruses expressing BMP9-transfected rDFCs (Ad-BMP9-transfected rDFCs). With this implant, we achieved more alveolar bone regeneration in the alveolar bone defect compared to blank group, CHA group and rDFCs group. Our results provided a novel bio-implants composed of Ad-BMP9-transfected rDFCs and CHA scaffolds and its mechanism is regarding the activation of Smad1/5/8 signaling pathway in BMP9-induced rDFCs osteogenesis.
细胞、支架和生长因子在骨再生中起着重要作用。骨形态发生蛋白 9（BMP9）是 BMP 家族的成员，通过调节生长因子和促进血管生成来促进成骨作用。与其他干细胞一样，大鼠牙囊干细胞（rDFCs）是成牙骨质细胞、成骨细胞和牙周膜细胞的前体细胞，可以自我更新并表现出多能性。珊瑚羟基磷灰石（CHA）具有良好的生物相容性和骨组织工程所需的导电性。在这里，我们报道 BMP9 可以增强细胞培养中 rDFCs 的成骨分化。此外，我们的结果表明 BMP9 通过 Smad1/5/8 信号通路发挥作用。我们还制备了一种新型支架，该支架包含生物可降解的 CHA 并接种了表达 BMP9 的重组腺病毒转染的 rDFCs（Ad-BMP9 转染的 rDFCs）。通过这种植入物，与空白组、CHA 组和 rDFCs 组相比，我们在牙槽骨缺损中实现了更多的牙槽骨再生。我们的结果提供了一种由 Ad-BMP9 转染的 rDFCs 和 CHA 支架组成的新型生物植入物，其机制是 BMP9 诱导的 rDFCs 成骨作用中 Smad1/5/8 信号通路的激活。
