Es-Haghi Ali, Mashreghi Mohammad, Rezazade Bazaz Mahere, Homayouni-Tabrizi Masoud, Darroudi Majid
Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
IET Nanobiotechnol. 2017 Aug;11(5):517-522. doi: 10.1049/iet-nbt.2016.0160.
The aim of this study is to introduce natural-based polymers, chitosan and starch, to design a remedial nanocomposite, comprising of cerium oxide nanoparticles and silver nanoparticles, to investigate their effects in accelerating wound healing and in wound microbial load. Cerium oxide nanoparticles synthesized in starch solution added to the colloidal dispersion of synthesized silver nanoparticles in chitosan to make a three-component nanomaterial. Mice were anaesthetized and two parallel full-thickness round wounds were excised under aseptic conditions with the help of sterile dermal biopsy punch. Furthermore, effects of silver-chitosan and silver-cerium-chitosan nanocomposite had evaluated on rate of wound closure and collagen density and on microbial load of wound in full-thickness model. Results showed that both silver chitosan and silver-cerium-chitosan had significant impact on acceleration of wound closure and collagen content and on reduction of wound microbial load in comparison with control group, which was, received no treatments. However, the silver-cerium-chitosan nanocomposite is more potent than silver-chitosan group and control group in wound closure. The wound healing effects of silver-cerium-chitosan nanocomposite are due to unique features of its three components and this nanocomposite promises impressive remedies for clinical application.
本研究的目的是引入基于天然的聚合物壳聚糖和淀粉，设计一种由氧化铈纳米颗粒和银纳米颗粒组成的修复性纳米复合材料，以研究它们在加速伤口愈合和减少伤口微生物负荷方面的作用。将在淀粉溶液中合成的氧化铈纳米颗粒添加到壳聚糖中合成的银纳米颗粒的胶体分散液中，制成一种三组分纳米材料。对小鼠进行麻醉，并在无菌条件下借助无菌皮肤活检打孔器切除两个平行的全层圆形伤口。此外，还评估了银 - 壳聚糖和银 - 铈 - 壳聚糖纳米复合材料对全层模型中伤口闭合率、胶原蛋白密度以及伤口微生物负荷的影响。结果表明，与未接受任何治疗的对照组相比，银 - 壳聚糖和银 - 铈 - 壳聚糖对加速伤口闭合、增加胶原蛋白含量以及降低伤口微生物负荷均有显著影响。然而，银 - 铈 - 壳聚糖纳米复合材料在伤口闭合方面比银 - 壳聚糖组和对照组更有效。银 - 铈 - 壳聚糖纳米复合材料的伤口愈合效果归因于其三种成分的独特特性，这种纳米复合材料有望为临床应用带来令人印象深刻的治疗方法。
